BMC Genomics

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A systematic survey in Arabidopsis thaliana of transcription factors that modulate circadian parameters

Shigeru Hanano1,3, Ralf Stracke1,2, Marc Jakoby1, Thomas Merkle2, Malgorzata A Domagalska1,4, Bernd Weisshaar1,2 and Seth J Davis1*

Author Affiliations

1 Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany

2 Bielefeld University, Department of Biology, Chair of Genome Research, D-33594 Bielefeld, Germany

3 Research Institute for Biological Sciences OKAYAMA Okayama 716-1241 Japan

4 Department of Biology, University of York. PO Box 373, York, YO10 5YW; UK

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BMC Genomics 2008, 9:182 doi:10.1186/1471-2164-9-182

Published: 21 April 2008

Additional files

Additional file 1:

Supplemental Table 1 – Circadian regulated MYB, bHLH and bZIP genes. Circadian expression values available in the public database GENEVESTIGATOR were scored for circadian regulation using the modified cosinor analysis program COSOPT. Mean of expression levels, period length, phase values (ZT) and pMMC-β are represented. COSOPT (pMMC-β < 0.05) without linear regression are listed here.

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Additional file 2:

Supplemental Table 2 – Estimated period length of transgenic lines overexpressing MYB transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.

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Additional file 3:

Supplemental Table 3 – Estimated period length of transgenic lines overexpressing bHLH transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.

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Additional file 4:

Supplemental Table 4 – Estimated period length of transgenic lines overexpressing bZIP transcription factors. Mean circadian periods of leaf movement in Arabidopsis plants misexpressing transcription factors and control seedlings, estimated with BRASS. S.E.M.: standard error of the mean, n: number of contributing leaf traces.

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Additional file 5:

Figure S1 – Confirmation of over-expression of MYB3R2 and bHLH69. Replicate seedlings from wild-type plants maintained under constant light were harvested every 4 hours. Total RNA was the substrate for RT-PCR of the coding regions of the transcription factors MYB3R2, bHLH69 and bHLH92. Results are presented as proportional to the average value after normalization with respect to TUB. (A) MYB3R2 and (B) bHLH69.

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Additional file 6:

Figure S2 – Confirmation of transcriptional clock phenotype of bHLH92-ox in constant light and in constant dark. Seedlings harboring CCA1:LUC reporter genes were monitored for 4–5 days both under LL (A) or in DD (B). Representative traces of rhythmic expression of ox-plants (pink squares) and wild-type (blue circles) are shown. (C) Relative Amplitude Error (R. A. E.) calculated from the data under LL was plotted against period (h). bHLH92-ox exhibited a slightly long periodicity phenotype.

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Additional file 7:

Figure S3 – Estimated period and phase of MYB3R2-ox, bHLH69-ox and bHLH92-ox. Estimated period length and phase values were calculated by BRASS. (A) Estimated period of CCA1 rhythm. (B) Peak positions of second peak in CCA1 rhythm. (C) Estimated period of CCR2 rhythm. (D) Peak positions of second peak in CCR2 rhythm. Data are presented as mean ± S.E. with n of 12–24 plants. * P = 0.01. No significant difference in periodicity was observed (A and C).

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Additional file 8:

Figure S4 – The effects of over-expression of MYB3R2 and bHLH69. Flowering time of MYB3R2-ox and bHLH69-ox plants was measured under long day. Leaf number at flowering time were plotted against the genotype and line tested. Data are presented as mean ± S.E. with n of 9–14 plants. * P = 0.038. No significant differences were detected in the flowering time of other lines.

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