Table 4

Relative abundance of selected miRNA reads in the control and Bj libraries^a

Library

Control

Bj

miRNA Family

Number of reads

Frequency^b

Number of reads

Frquency^b

Relative abundance (Bj/Control)

171^d

12

0.18

23

0.27

1.5

482

21

0.31

39

0.46

1.5

1507

22

0.33

38

0.44

1.4

390

12

0.18

19

0.22

1.3

160

39

0.58

60

0.70

1.2

1509

216

3.21

318

3.72

1.2

1508

28

0.42

40

0.47

1.1

396

54

0.80

77

0.90

1.1

159

584

8.68

831

9.73

1.1^c

164

25

0.37

35

0.41

1.1

166

50

0.74

68

0.80

1.1

168

29

0.43

39

0.46

1.1

393

30

0.45

39

0.46

1.0

894?

7

0.10

9

0.11

1.0

1510

23

0.34

29

0.34

1.0

156

37

0.55

46

0.54

1.0

171

60

0.89

72

0.84

1.0

1511

11

0.16

13

0.15

0.9

167

52

0.77

61

0.71

0.9

1512

8

0.12

9

0.11

0.9

172

184

2.74

205

2.40

0.9

482?

20

0.30

22

0.26

0.9

169

31

0.46

31

0.36

0.8

162

6

0.09

6

0.07

0.8

319

50

0.74

45

0.53

0.7

1513

25

0.37

20

0.23

0.6

^a Only reads with a total abundance of ≥ 10 in both libraries combined are presented.

^b Frequency is calculated as the number of reads representing the family per thousand reads in the corresponding library that matched known soybean sequences. Number of reads in Control and Bj libraries that matched known soybean sequences were 67248 and 85406 respectively.

^cThe difference in read counts is statistically significant (P < 0.05) based on Poisson's distribution analysis.

^d The precursors of these miRNAs seems to be evolutionarily related, but the mature miRNAs are different from each other and we classified them as a novel miRNA.

Subramanian et al. BMC Genomics 2008 9:160   doi:10.1186/1471-2164-9-160