Microarray analysis of E9.5 reduced folate carrier (RFC1; Slc19a1) knockout embryos reveals altered expression of genes in the cubilin-megalin multiligand endocytic receptor complex

Janee Gelineau-van Waes¹ , Joyce R Maddox¹ , Lynette M Smith² , Michael van Waes³ , Justin Wilberding¹ , James D Eudy¹ , Linda K Bauer¹ and Richard H Finnell⁴

¹Department of Genetics, Cell Biology & Anatomy, University of Nebraska Medical Center, Omaha, NE 68198-5455, USA

²Department of Preventive and Societal Medicine, University of Nebraska Medical Center, Omaha, NE 68198-4350, USA

³LI-COR Biosciences Inc., Lincoln, NE 68504, USA

⁴Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030, USA

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BMC Genomics 2008, 9:156doi:10.1186/1471-2164-9-156


Published:	9 April 2008

Additional files

Additional file 1:

RFC1 microarray gene table. This table provides a complete list of the 200 known genes that were differentially expressed between E9.5 RFC1 nullizygous and wildtype embryos following statistical analysis of the microarray data, including RefSeq transcript ID, gene symbol, description, and fold change for each gene.

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Additional file 2:

Comparison of mouse mutants. This table compares the phenotype of RFC1 nullizygous embryos (on low or high dose maternal folate supplementation) with the phenotype of embryos in which different genes in the cubilin-megalin multiligand endocytic receptor complex (ie. cubilin, amnionless, megalin, Folr1, Dab2, Lrpap1) have been inactivated.

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Additional file 3:

Quantitative RT-PCR primer/probe list. This table contains a complete list and details regarding the primers/probes used in the qRT-PCR analysis of genes in the multiligand endocytic receptor complex, as well as details on the function of each gene examined.

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