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Oligonucleotides used to generate serial 5'-deletions of the rat and human HL promoter region. |
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| Name |
species and orientationa |
positionb |
5'→3' sequencec |
|
|
|
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| 3F |
r |
F |
-1697/-1675 |
cggaagc TTA GCA GAC AGC GAT TGG C |
| 4F |
r |
F |
-1048/-1030 |
cggaag CTT GCC TCC TCC TGA GTG C |
| 5F |
r |
F |
-754/-736 |
cggaagc TTG TCC AGG GCG TCC ATA C |
| 9F |
r |
F |
-211/-196 |
cggaagctt AGC TTG GCT CAA AAG G |
| 8F |
r |
F |
-86/-71 |
cggaagctt GTG TTC AAA TAC TGG G |
| 7F |
r |
F |
-75/-58 |
cggaagct TGG GTA ACA TGT TTT AGG |
| 6F |
r |
F |
-39/-22 |
cggaag CTT CCA CAA CTA AAT ACC |
| 11F |
r |
F |
-23/-8 |
cggaagctt CCA AGA AGC ATT CTG G |
| HHL-685Sac |
h |
F |
-685/-667 |
ccgagctc TGG TCG CCT TTT CCC TAC C |
| HHL-306Nhe |
h |
F |
-306/-291 |
gcatgctagc GAA GCC ACC TAC CCC G |
| HHL-79Kpn |
h |
F |
-79/-55 |
ggggtacc TAA CAT GTT GAG AGG |
| HHL-36Kpn |
h |
F |
-36/-20 |
ggggtac CAA AGT ATC TAA TAG GC |
| HHL+13Xba |
h |
R |
+13/-6 |
gctctaga CTT GGT AAT TTC TGA AGC C |
| HHL-10Kfw |
h |
F |
-10457/-10437 |
gtcgac GAA GGA TCA GGT GAG GGA TGG |
| HHL-10Krev |
h |
R |
-9956/-9975 |
ggatcc GCT TCA AGG GCA ATG AAA GC |
| HHL-14Kfw |
h |
F |
-14202/-14183 |
gtcgac GAC TTG GGG ATA TCC ACA TC |
| HHL-14Krev |
h |
R |
-13524/-13549 |
ggatc CTG GTA AAA GGA CAT GAA CAA TAT GG |
| HHL-22Kfw |
h |
F |
-21929/-21909 |
gtcgac GAT GGG AAA TGG ACC TAC AGC |
| HHL-22Krev |
h |
R |
-21543/-21562 |
ggatcc GAT GAG GAC TGA TTC TCA GC |
| CATREV2d |
- |
R |
gca act gac tga aat gcc tc |
|
| T3primere |
- |
R |
att aac cct cac taa ag |
|
|
a: r and h: rat and human, respectively; F and R: forward and reverse orientation. b: numbering of the rat and human sequence according to [35] and Ensembl e!42 [26], respectively. c: rat and human HL specific parts of the primer sequences are given in capitals. d: oligonucleotide specific for pCAT-Basic. e: oligonucleotide specific for pBluescript. | ||||
van Deursen et al. BMC Genomics 2007 8:99 doi:10.1186/1471-2164-8-99 |
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