Hierarchical clustering of genes regulated by TGF-β in (a) HPL1D and (b) A549 cells. The genes regulated by TGF-β (described in materials and methods) were clustered using Avadis 3.3 Prophetic software. The data was clustered on rows by one way complete linkage – hierarchical clustering using Euclidean distance metric. Microarrays have been done in duplicate on RNAs isolated from cells after 1, 4 and 12 hours of treatment with TGF-β with paired controls at every time point. The dendrogram on the left shows the different clusters into which the TGF-β regulated genes segregated. Red indicates genes induced by TGF-β treatment, green indicates genes repressed by TGF-β treatment and black suggests no regulation.
Ranganathan et al. BMC Genomics 2007 8:98 doi:10.1186/1471-2164-8-98