Computational prediction and experimental validation of Ciona intestinalis microRNA genes

Trina M Norden-Krichmar¹ , Janette Holtz² , Amy E Pasquinelli² and Terry Gaasterland¹

¹Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, MSC 0202, La Jolla, CA 92093 USA

²Department of Biology, University of California, San Diego, La Jolla, CA 92093 USA

author email corresponding author email

BMC Genomics 2007, 8:445doi:10.1186/1471-2164-8-445


Published:	29 November 2007

Additional files

Additional File 1:

Analysis of the characteristics of known miRNAs. This file summarizes the miRNA statistics gathered for three pairs of closely related organisms: Caenorhabditis elegans vs. Caenorhabditis briggsae, Drosophila melanogaster vs. Drosophila pseudoobscura, and Homo sapiens vs. Pan troglodytes.

Format: PDF Size: 346KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 2:

List of predicted miRNAs for C. intestinalis and their corresponding probe sequences. This file lists the putative C. intestinalis miRNAs, their mature miRNA sequence, oligonucleotide probe sequence, scaffold number, coordinates, strand, position, and nearest neighboring gene.

Format: XLS Size: 28KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional File 3:

List of predicted miRNA targets for C. intestinalis. This file contains a list of the predicted targets with single and multiple hits to the 3'UTR region.

Format: XLS Size: 41KB Download file

This file can be viewed with: Microsoft Excel Viewer