Methodology to remove regions of tandem codon repetition. Repetitions of more than 3 consecutive codons were excluded from the analysis by analyzing four consecutive codons at each step. At each iteration, the presence of identical codons forming 4 consecutive triplets was verified and when such triplets were found the algorithm proceeded reading without counting the triplets until a different codon appeared in the ORF sequence.
Moura et al. BMC Genomics 2007 8:444 doi:10.1186/1471-2164-8-444