|
Real-time PCR validation of microarray negative results |
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| Gene* (Clone No. on Microarray) |
Hog 1/hog 2 |
Sow 1/sow 2 |
Hog 3/hog 4 |
Sow 3/sow 4 |
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|
|
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| Change (test/control) |
P value |
Change (test/control) |
P value |
Change (test/control) |
P value |
Change (test/control) |
P value |
|
|
|
||||||||
| SCD (rpfat_16685) |
1.56 |
0.1680 |
0.77 |
0.2119 |
1.49 |
0.0822 |
1.23 |
0.9943 |
| HSL (rpfat_11096) |
1.29 |
0.0679 |
1.11 |
0.2989 |
1.29 |
0.3462 |
1.06 |
0.5294 |
| PMP22a (rpfat_18575) |
1.15 |
0.6198 |
1.16 |
0.3178 |
1.24 |
0.6900 |
1.46 |
0.1175 |
| PHPT1b (rpfat_15312) |
0.75 |
0.1218 |
0.95 |
0.5811 |
1.24 |
0.4694 |
1.46 |
0.4869 |
|
* The full names of each gene are listed in Tables 2-5. a : PMP22: peripheral myelin protein 22 b :PHPT1: Phosphohistidine phosphatase 1 Three genes (HSL, PMP22 and PHPT1) were confirmed by real-time PCR. | ||||||||
Zhang et al. BMC Genomics 2007 8:433 doi:10.1186/1471-2164-8-433 |
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