Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design

doi:10.1186/1471-2164-8-423

BMC Genomics

Volume 8

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Ramsey JS
Wilson AC
de Vos M
Sun Q
Tamborindeguy C
Winfield A
Malloch G
Smith DM
Fenton B
Gray SM
Jander G

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Ramsey JS
Wilson AC
de Vos M
Sun Q
Tamborindeguy C
Winfield A
Malloch G
Smith DM
Fenton B
Gray SM
Jander G
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Research article

Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design

John S Ramsey¹ , Alex CC Wilson²^,3 , Martin de Vos¹ , Qi Sun⁴ , Cecilia Tamborindeguy⁵ , Agnese Winfield⁶ , Gaynor Malloch⁶ , Dawn M Smith⁵ , Brian Fenton⁶ , Stewart M Gray⁵ and Georg Jander¹

¹Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA

²Department of Biology, University of Miami, Coral Gables, Florida 33146, USA

³Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA

⁴Cornell Theory Center, Cornell University, Ithaca, NY 14853, USA

⁵USDA/ARS, Plant Protection Research Unit, Ithaca, NY 14853, USA

⁶Scottish Crop Research Institute, Invergowrie, Dundee, UK

author email corresponding author email

BMC Genomics 2007, 8:423doi:10.1186/1471-2164-8-423


Published:	16 November 2007

Additional files

Additional file 1:

Collection and life cycle characterization of aphid lineages. This table provides information on aphids collected for this study, including host plant and site of collection, and results of life cycle characterization.

Format: XLS Size: 27KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 2:

BlastX comparisons of M. persicae contigs to GenBank DNA sequences. This table provides a working annotation of unigenes described in this study, based on BlastX comparisons to GenBank DNA sequences.

Format: XLS Size: 6.4MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 3:

Full listing of M. persicae ESTs, along with the source cDNA libraries. This table lists accession numbers of all ESTs sequenced in this study, in association with the contig to which they belong and the library from which they are derived.

Format: XLS Size: 3.2MB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 4:

Secreted proteins in salivary glands, as predicted by SignalP 3.0. This table provides annotation and signal peptide prediction information for unigenes overrepresented in the salivary gland library.

Format: XLS Size: 58KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 5:

Potential SNPs identified based on sequence differences among M. persicae lineages. This table lists SNPs identified among the sequences generated in this study by PolyBayes with a probability cutoff of 0.84.

Format: XLS Size: 197KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 6:

High-confidence SNPs, represented by at least two reads in at least two M. persicae lineages. This table provides a list of filtered SNPs likely to represent polymorphisms between M. persicae lineages.

Format: XLS Size: 51KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional file 7:

PCR primers used in this study. This table lists the sequences for all PCR primers used in this study.

Format: XLS Size: 27KB Download file

This file can be viewed with: Microsoft Excel Viewer