Table 5

Results from the rcPCR bootstrap statistics (x4000), Landrace

Gene

Fold

Log10 Fold change

Bias

Std error

P value


SMPD1

1.4

0.16

-1.60

0.18

0.185

StAR

12.8

1.11

-2.65

0.13

0.000

SULT2A1

2.4

0.38

-4.37

0.11

0.002

HSD17B4

1.7

0.23

-2.13

0.09

0.006

AKR1C4

2.5

0.39

-2.07

0.09

0.000

CYP11A1

3.2

0.50

5.84

0.12

0.000


rcPCR was used to confirm expression variations of selected genes from the microarray study. Fold changes are calculated relative to baseline, which is the group of low androstenone (NLL) in this case, and adjusted for the housekeeping gene HPRT. Five of six examined genes were verified and one was contradicted.

Moe et al. BMC Genomics 2007 8:405   doi:10.1186/1471-2164-8-405

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