Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-freeBMP4 culture

doi:10.1186/1471-2164-8-365

BMC Genomics

Volume 8

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Research article

Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-free^BMP4culture

Stephen J Bruce¹^,2 , Brooke B Gardiner¹^,3^,4 , Les J Burke¹^,4 , M Milena Gongora¹^,3 , Sean M Grimmond¹^,3^,4 and Andrew C Perkins¹^,2^,3^,4

¹Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, Australia

²Wesley Research Institute, Queensland, Australia

³ARC Special Research Centre in Functional and Applied Genomics, Australia

⁴Australian Stem Cell Centre, Melbourne, Australia

author email corresponding author email

BMC Genomics 2007, 8:365doi:10.1186/1471-2164-8-365


Published:	10 October 2007

Additional files

Additional file 1:

Oct4 gene list. The data provided lists all genes expressed during 16 days of embryoid body differentiation with similarity to Oct4 (Pearson correlation >0.9).

Format: DOC Size: 630KB Download file

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Additional file 2:

Nanog gene list. The data provided lists all genes expressed during 16 days of embryoid body differentiation with similarity to Nanog (Pearson correlation >0.9).

Format: DOC Size: 111KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 3:

Sox2 gene list. The data provided lists all genes expressed during 16 days of embryoid body differentiation with similarity to Sox2 (Pearson correlation >0.9).

Format: DOC Size: 237KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 4:

Sequence alignment of RIKEN clone 8430415E04RIK. The data provides an alignment of RIKEN clone 8430415E04RIK using human, mouse, chick and zebrafish sequences.

Format: DOC Size: 31KB Download file

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Additional file 5:

FGF5 gene list. The data provided lists all genes expressed during 16 days of embryoid body differentiation with similarity to FGF5 (Pearson correlation >0.9).

Format: DOC Size: 69KB Download file

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Additional file 6:

Wnt5a gene list. The data provided lists all genes expressed during 16 days of embryoid body differentiation with similarity to Wnt5 (Pearson correlation >0.9).

Format: DOC Size: 76KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 7:

Position Weighted Matrices. Provides published data on position weighted matrices for Oct, Sox, KLF-A, KLF4, Nanog, E-box, Gata-1 and their variants.

Format: DOC Size: 116KB Download file

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Additional file 8:

Oligonucleotide sequences used for qRT-PCR analysis. Lists the oligonucleotide sequences and cycling conditions used for the qRT-PCR analysis.

Format: DOC Size: 48KB Download file

This file can be viewed with: Microsoft Word Viewer