Open Access Research article

Cadmium triggers an integrated reprogramming of the metabolism of Synechocystis PCC6803, under the control of the Slr1738 regulator

Laetitia Houot1, Martin Floutier12, Benoit Marteyn1, Magali Michaut1, Antoine Picciocchi1, Pierre Legrain12, Jean-Christophe Aude1, Corinne Cassier-Chauvat12 and Franck Chauvat1*

Author Affiliations

1 Commissariat à l'Energie Atomique, Institut de Biologie et Tecnologies de Saclay, Service de Biologie Intégrative et Génétique Moléculaire, CEA Saclay F-91191 Gif sur Yvette CEDEX, France

2 Centre National de la Recherche Scientifique, Unité de Recherche Associée 2096 CEA Saclay, F-91191 Gif sur Yvette CEDEX, France

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BMC Genomics 2007, 8:350  doi:10.1186/1471-2164-8-350

The electronic version of this article is the complete one and can be found online at:

Received:8 June 2007
Accepted:2 October 2007
Published:2 October 2007

© 2007 Houot et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.



Cadmium is a persistent pollutant that threatens most biological organisms, including cyanobacteria that support a large part of the biosphere. Using a multifaceted approach, we have investigated the global responses to Cd and other relevant stresses (H2O2 and Fe) in the model cyanobacterium Synechocystis PCC6803.


We found that cells respond to the Cd stress in a two main temporal phases process. In the "early" phase cells mainly limit Cd entry through the negative and positive regulation of numerous genes operating in metal uptake and export, respectively. As time proceeds, the number of responsive genes increases. In this "massive" phase, Cd downregulates most genes operating in (i) photosynthesis (PS) that normally provides ATP and NADPH; (ii) assimilation of carbon, nitrogen and sulfur that requires ATP and NAD(P)H; and (iii) translation machinery, a major consumer of ATP and nutrients. Simultaneously, many genes are upregulated, such as those involved in Fe acquisition, stress tolerance, and protein degradation (crucial to nutrients recycling). The most striking common effect of Cd and H2O2 is the disturbance of both light tolerance and Fe homeostasis, which appeared to be interdependent. Our results indicate that cells challenged with H2O2 or Cd use different strategies for the same purpose of supplying Fe atoms to Fe-requiring metalloenzymes and the SUF machinery, which synthesizes or repairs Fe-S centers. Cd-stressed cells preferentially breakdown their Fe-rich PS machinery, whereas H2O2-challenged cells preferentially accelerate the intake of Fe atoms from the medium.


We view the responses to Cd as an integrated "Yin Yang" reprogramming of the whole metabolism, we found to be controlled by the Slr1738 regulator. As the Yin process, the ATP- and nutrients-sparing downregulation of anabolism limits the poisoning incorporation of Cd into metalloenzymes. As the compensatory Yang process, the PS breakdown liberates nutrient assimilates for the synthesis of Cd-tolerance proteins, among which we found the Slr0946 arsenate reductase enzyme.


Photosynthetic organisms that support much of the life on Earth, in using solar energy to renew the oxygenic atmosphere and make up organic assimilates essential to the food chain [1,2] are frequently challenged with toxic

pecies (ROS) generated by respiration and photosynthesis [3], and toxic metals that constitute persistent pollutants because they cannot be degraded. One of them, Cadmium (Cd), is very abundant in the environment as it is often combined with sulfur in Earth's crust, and it is also intensively spread out as (i) a by-product of zinc mining, (ii) the burning of fossil fuel, (iii) the dispersal of sewage sludge and phosphate fertilizers, and (vi) the manufacturing of paints, batteries and screens [4]. Subsequently, Cd can be transferred to the food chain, and bio-accumulated in human where it has a half-life greater than 20 years [5] and causes various diseases by as yet unclear processes [6]. Even metals that are essential to enzyme activity, such as zinc and iron [7,8], can become toxic when occurring in excess. This toxicity is likely due to the poisoning replacement of the cognate metal cofactor of diverse metalloenzymes, a phenomenon sometimes leading to oxidative stress [9].

Cyanobacteria, the most abundant photosynthetic organisms on Earth [10], are attractive models to investigate the interrelations between metal toxicity and oxidative stress, because they perform the two metal-requiring [8] ROS-generating processes [3], photosynthesis and respiration, in the same membrane system [11]. Furthermore, cyanobacteria share a wide range of genes in common with plants [12], in agreement with they being the likely ancestor of chloroplast [13]. Thus, lessons learned from stress responses in cyanobacteria will also greatly facilitate the understanding of how plant cells face environmental challenges. This is important, as Cd has been reported to be toxic to plants by as yet unknown processes that may [14] or may not [15] impair photosynthesis. Moreover, cyanobacteria are also suitable for biosensor and/or bioremediation applications [16,17].

Using the model cyanobacterium Synechocystis PCC6803 that possesses a small genome [18] fully sequenced, and easily manipulable with replicating plasmids [19-21], we have analyzed the global responses of photosynthetic cells challenged with Cd, H2O2 (the paradigm ROS agent) or drastic changes of availability of either Fe or Zn, through (i) DNA microarrays; (ii) absorption spectroscopy; (iii) oxygen evolution; (iv) Western blot; (v) targeted gene inactivation and (vi) assays of cell fitness. We show that Cd triggers a "Yin Yang" integrated reorganization of the cyanobacterial metabolism, under the control of the Slr1738 regulator. The "Yin" ATP-sparing downregulation of cell metabolism likely limits Cd uptake and poisoning incorporation in place of the cognate metal cofactor of metalloenzymes. The compensatory "Yang" breakdown of the photosynthetic machinery that impairs ATP production, liberates nutrient assimilates that become available for the synthesis of Cd-toxicity protecting enzymes, among which we found the Slr0946 arsenate reductase.


Transcriptional regulations elicited by Cd are slower and more sustained than those triggered by H2O2

The transcriptome approach was used to characterize the kinetics of global changes in Synechocystis PCC6803 (Synechocystis) gene expression elicited by noxious agents, which were continuously applied to the cells to mimic the persistent character of stresses encountered in Nature. Exponentially growing cells were exposed to CdSO4 (50 μM) or H2O2 (3 mM) for increasing periods of time that triggered a wide range of changes in cellular viability (from 100% to less than 10%) and regulation (number of responsive genes and magnitude of changes in expression), as required for a thorough investigation of stress responses (Table 1). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labeled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point), as described in Methods. Our data (Table 1 and see Additional files 1, 2, 3, 4) showed that the Cd-elicited regulation could be divided in two main temporal phases. The early phase was moderate since only 151 genes responded to Cd during the first 60 min of treatment, and the changes were mostly up-regulation. The second phase occurring between 90 to 360 min of treatment was massive, with about 1,222 responsive genes equally distributed between up- and down-regulated genes. The relevance of the "early" and "massive" phases of Cd responses was verified by performing an independent biological repeat of both the time points 60 min (early phase) and 300 min (massive phase), and using an appropriate statistical test (Methods) to analyze all data (see Additional files 2, 3). Indeed, a large number of the Cd-regulated genes (791) appeared to be differentially expressed between the two temporal phases of responses.

Additional file 1. Analysis of the unstressed versus unstressed control experiment. These file describe the distribution of allexpression ratio for the unstressed versus unstressed control experiment, showing the extreme, mean and quantile values.

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Additional file 2. Kinetic analysis of the transcriptional responses to cadmium. The data provided indicate for each gene (column 1), encoding the indicated protein (column 12), the log-ratio of response to the indicated duration of the cadmium treatment (columns 2 to 10) and the p-value obtained by the analysis (column 11).

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Additional file 3. Kinetic analysis of the transcriptional responses to hydrogen peroxide. The data provided indicate for each gene (column 1), encoding the indicated protein (column 8), the log-ratio of response to the indicated duration of the hydrogen peroxide treatment (columns 2 to 6), and the p-value obtained by the analysis (column 7).

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Additional file 4. Relevant List of Stress-Responsive Genes. The data provided indicate for each gene (extreme left and right columns), sorted by the physiological function they operate in, the log-ratio of response to the indicated duration of stresses.

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Table 1. Influence of Stress on the Viability and Global Transcription Profile

By contrast, the transcriptional responses to H2O2 (3 mM) were faster and briefer than those to Cd (Table 1 and see Additional files 2, 3, 4). The massive phase of H2O2-mediated regulation encompassed the time points 15 min and 30 min (1,300 genes controlled, equally distributed between up- and down-regulation), while the late phase occurred between 180 min and 420 min (344 genes controlled, mostly positively), a time period in which most fast-responsive genes had returned to normal expression level (see Additional file 3).

Cadmium antagonistically controls the genes operating in protein synthesis (downregulation), and protein maturation and degradation (upregulation)

Among the earliest responses to Cd (noticeable within the first 30 min of exposure) was the upregulation (see Additional file 4 panel A) of chaperones and proteases genes, the number of which increased during the massive phase of responses (after 60 min.). This regulation was accompanied with the downregulation of most ribosomal proteins genes (noticeable at 90 min, see Additional file 4 pannel A), and, comforting our data, we noticed that operonic genes were co-regulated. By contrast, most aminoacyl-tRNA synthetases genes were unaffected by Cd (see Additional files 2 and 6). Considering the normal level of expression [22] and the response to Cd (this study) of aminoacyl-tRNA synthetases genes (moderate expression, unresponsive to Cd) and ribosomal proteins genes (strong expression, turned down by Cd), we think that Cd-challenged cells preferentially downregulate those genes whose expression represents a metabolic burden. This interpretation is comforted by the findings that photosynthesis genes normally expressed to a high level [22] were also turned down by Cd (see below).

Similarly, H2O2 downregulated ribosomal protein genes (see Additional file 4, panel A), and did not affect aminoacyl-tRNA synthetase genes (see Additional file 3).

Also interestingly, we found that Zn excess partly mimics the Cd-mediated control of genes involved in protein folding and turnover (upregulation) or protein synthesis (downregulation), which were little affected by Fe availability (Table 1 and see Additional file 4).

Cd and to a lesser extent H2O2 downregulate photosynthesis genes

A very important target of Cd toxicity was the photosynthesis (PS) machinery that uses several electron-transfer complexes, the photosystemII (PSII) and its phycobilisomes (PBS) antennae, the cytochrome b6/f, the photosystemI (PSI) and the ATPase, to produce ATP and NADPH [8] required for the assimilation of inorganic nutrients. In addition, the PS activity can also generate toxic

pecies (ROS) in case of light excess [23]. Most of these PS genes were downregulated after 75–90 min. of Cd challenge (see Additional file 4 pannel B). The validity of these data was substantiated by the observed co-regulation of the following operons psaAB, psbCD1, apcABC and atpIHGFDACBE. In addition, we also observed the well-known [24] antagonistic iron regulation of the ssl0020 ferredoxin gene (repressed by Fe starvation) and isiB flavodoxin gene (induced by Fe limitation). Furthermore, we have verified the Cd-elicited downregulation of the psaC gene at the level of protein abundance (Fig. 1). Also consistent with the Cd-elicited downregulation of PS genes, we found (see Additional file 4 pannel B) that Cd (i) repressed most pigment synthesis genes, namely: hemA, hemL, hemB, hemE, hemF, hemN, chlN, chlB, chlL, por, ho1, ho2, cbiX, crtH, crtR, crtD-homolog and alg-homolog, and (ii) induced the nblA operonic genes that operate in PBS degradation [25].

thumbnailFigure 1. Influence of cadmium on the abundance of selected proteins. Cells were incubated for the indicated durations on solid media with or without (0 for untreated control) CdSO4 (50 μM, 360 min.) prior to disruption. 5 μg of crude cell extracts were analyzed by Western blottings (Methods), using the antibodies directed against the indicated proteins.

The global downregulation of PS and pigment synthesis genes has been observed in cells challenged by a high light stress [26-28] and, very interestingly, we noticed that many of the high light-inducible genes [25,29,30] were also upregulated by Cd (see Additional file 4 panel B) namely: hliB, hliC, isiA and nblA. Collectively, these findings suggested that Cd-exposed cells become light sensitive, an interpretation we validated through growth assays (Fig. 2C).

thumbnailFigure 2. Effect of metal, hydrogen peroxide and light fluence on cellular growth. Four fold serial dilutions of mid log phase liquid cultures were spotted onto BG11 plates with or without the indicated agents, incubated for 4–5 days and scanned (Methods). Panel A: Typical influence of iron ((NH4)FeH2C6H5O7 or FeCl3), cobalt (Co(NO3)2) and citrate ((NH4)2H2C6H5O7) on the toxicity of cadmium (CdSO4). Panel B: Typical influence of iron ((NH4)FeH2C6H5O7) on the toxicity of hydrogen peroxide (H2O2). Panel C: Influence of the light fluence on the toxicity of CdSO4. These experiments were repeated three times.

The aspects of Cd toxicity resembling light stress are presumably due to oxidative stress since they could be elicited by H2O2 too (see Additional file 4 panel B). These common responses included the downregulation of all ATPase genes and several PBS genes (apcA, apcB, apcC, apcE and apcF), as well as the concomitant upregulation of many high light-inducible genes (hliC, isiA and nblA). Unsurprisingly, H2O2 mimicked high light stress that generates ROS [3] more efficiently than Cd. Indeed (see Additional file 4 panel B), H2O2 upregulated several high light-inducible genes unaffected by Cd, namely: hliA, hliD, ctpA and ftsH (slr0228 and slr1604) the protease genes involved in the high-light induced turnover of the D1 protein of PSII [31]. Also interestingly, many PS genes downregulated by Cd were actually upregulated by H2O2, namely: PSII (psbB, psbJ, psbV and psbU), PSI (psaF, psaJ, psaD, psaI, psaM) and PBS (cpcC1, cpcC2 and cpcD) (see Additional file 4 panel B).

In agreement with the upregulation of many genes induced by high light that triggers oxidative stress, we found (see Additional file 4 panel D) that Cd and/or H2O2 upregulated many anti-oxidant genes encoding thioredoxin reductase (trxR), thioredoxins (trxA, trxM), glutathione peroxidase (gpx), glutaredoxins (grx), glyoxalases (glo) and peroxidases (gpx and ahpc).

Similarly to Cd, Zn downregulated numerous PS genes (PBS, PSII, PSI and pigment synthesis, but not ATPase genes), and upregulated genes involved in protein turnover and tolerance to light/oxidative stress (see Additional file 4 panel B). By contrast, Fe controlled a few PS genes.

Also interestingly, the differential regulation of the cytochrome b6/f genes (TableS4B), encoding the predominant (petC1) or accessory (petC2 and petC3) Rieske iron-sulfur proteins [32], strongly suggests that alternative b6/f complexes are synthesized in response to changing environmental conditions.

Spectroscopic confirmation that Cd elicits a more intense decline of the photosynthetic machinery than H2O2

That Cd- and Zn-excess turned down most photosynthesis genes and simultaneously upregulated protein degradation genes, suggested to us that these stresses decrease the abundance of the PS machinery. By comparison, we anticipated H2O2 to elicit a lower decline of the PS apparatus, in downregulating a smaller number of PS genes (see above). These predictions were all validated by another global method i.e. absorption-spectroscopy, which showed that the cellular content of colored PS pigments was decreased strongly in response to Cd- and Zn-stresses (Fig. 3A and 3B) and weakly in response to H2O2 (Fig. 3D). As control experiments, we have verified that excess of Fe (with little influence PS-gene expression see above) or cobalt did not alter pigments content (Fig. 3C and 3F).

thumbnailFigure 3. Influence of metals and H2O2 on the abundance of photosynthetic pigments. Typical absorption spectra of the wild type (WT) or slr1738 null-mutant (Δslr1738) cells following incubation for the indicated durations on solid BG11 medium with or without H2O2 (3 mM), CdSO4 (50 μM), Co(NO3)2 (350 μM), (NH4)FeH2C6H5O7 (350 μM) or ZnSO4 (350 μM or 776 μM). The spectra (normalized to light scattering at 800 nm) are displayed in panels A to F. These experiments were repeated three to five times.

Oxygen evolution confirmation that Cd impairs photosynthesis

To further demonstrate that Cd impairs photosynthesis we measured the rate of the whole photosynthetic electron transport (from H2O2 to CO2) of intact cells incubated with or without 50 μM Cd. As expected, the oxygen-evolving activity of Cd-treated cells was strongly decreased (2.5- and 7-fold after 3- and 6-h, respectively) as compared to that of untreated cells.

Cd and H2O2 likely disturb metal homeostasis

Cd rapidly and continuously altered expression of numerous metal transport genes, indicating that it disturbs metal homeostasis (see Additional file 4 panel C). For instance, all members of the nine genes cluster involved in the tolerance to Ni (nrsBACD operon), Co (coaRT divergon, sll0794 and slr0797) and Zn (ziaBR operon and ziaA export ATPase) were upregulated by Cd. As one of the numerous findings attesting the relevance of our transcriptome data we observed (see Additional file 4 panel C) that Zn controlled the genes znuA (slr2043, Zn uptake, downregulation) and ziaA (Zn export, upregulation), as expected [24,33,34]. That Cd regulated both znuA (negatively) and ziaA (positively), whose product is homologous to the Cd-transporting ATPase CadA [35], suggesting that Cd might be transported via Zn transport systems. Cd also controlled the corR-corT divergon operating in Co efflux, as well as the cbi cluster and the cbiX gene involved in the biosynthesis of cobalamin the Co-dependent vitamin B12 [36]. These data suggest that Cd disturbs Co homeostasis and utilization.

A large part of the numerous genes (more than 20) dedicated to Fe acquisition (feoB, fec, fhu and fut) were found to be positively regulated by Fe starvation and turned down by Fe excess (see Additional file 4 pannel B), in agreement with previous Northern blot data [37]. Again, attesting the relevance of our data, we also observed the Fe starvation-mediated control (see Additional file 4 panel D) of the isiAB operon (upregulation) and the fed1 genes (downregulation), as expected [38].

H2O2 upregulated all Fe acquisition genes (see Additional file 4 pannel C), as well as (see Additional file 4 panel D) the suf genes involved in iron-sulfur cluster biogenesis [39]. These findings are reminiscent to what occurs in E. coli where oxidative-stressed cells induce Fe uptake and suf genes to accelerate the supply of Fe atoms for the reconstitution of damaged iron-sulfur clusters, in a process leaving no free Fe atoms available for the toxic Fenton chemistry [40-42]. Interestingly, Cd upregulated antioxidant and suf genes, as well as half the number of the Fe-uptake genes (see Additional file 4 panels C and D). These findings suggest that Cd damages Fe-S centers, and that the extra Fe atoms required for their repair might be provided not only by the presumably moderate increase in Fe uptake, but also by the breakdown of the Fe-rich photosynthetic machinery (Fig. 3A) that contains 21–23 iron atoms per PS unit [43].

Iron availability controls the Cd-elicited decline of cell viability and PS machinery

The above-mentioned data led us to predict that Fe availability can influence not only cell tolerance to H2O2 and Cd, but also the Cd-elicited decline of the PS machinery. As anticipated, we found that the addition of Fe in the medium at the onset of the stresses increased cell resistance to H2O2 and Cd (Fig. 2A and 2B), and prevented the Cd-elicited decline of the PS machinery (Fig. 3C). As a negative control, we verified that cobalt (Co) was unable to mimic these Fe-mediated protection effects (Fig. 2A and Fig. 3F).

The Slr0946 arsenate reductase contributes to cadmium tolerance

We noticed that the arsBHC tricistronic operon (slr0944 to slr0946) operating in arsenic resistance [44,45] was rapidly and continuously upregulated by Cd (see Additional file 4 panel C). To confirm that the ArsC arsenate-reductase enzyme is a key factor in the tolerance to cadmium, we have deleted the arsC gene (Methods), and found the corresponding fully-viable arsC null mutant to be more sensitive to Cd than the WT strain (Fig. 4).

thumbnailFigure 4. Influence of cadmium on the growth of the wild type strain and ΔarsC mutant. Typical growth of the wild type (WT, triangles) and ΔarsC mutant (circles) cultivated in liquid (PanelA) or solid (PanelB) BG11 media with or without CdSO4 (at the indicated concentration). These experiments were repeated three times.

Cd and H2O2 downregulate carbon metabolism genes, many of which encode ATP-requiring enzymes


echanism (CCM) genes for the acquisition and assimilation of
arbon (Ci) [46] were downregulated by Cd (see Additional file 4 pannel E), namely: (i) the ndhF3, ndhD3 and cupA tricistronic operon (CO2 uptake, NDH-I3 system); (ii) the ndhF4 and ndhD4 operon and the cupB gene (CO2 uptake, NDH-I4 system); (iii) the cmpABCD operon (HCO3- transporter); (iv) the sbtAB operon (HCO3- transporter); (v) the cca gene (carbonic anhydrase); (vi) the carboxysome genes ccmK4 and ccmK-N operon; (vii) the prk gene (phosphoribulokinase) and (viii) the ppc gene (phosphoenol pyruvate carboxylase). These results, together with the constitutive expression of the low-Ci inducible gene ndhR encoding the Ci-assimilation regulator [47], indicate that Cd challenged cells are not suffering from Ci starvation. Similarly, most carbon metabolism genes were turned down by Cd (see Additional file 4 panel E), namely: (i) gpmA (phosphoglycerate mutase); (ii) eno (enolase); (iii) pyk2 (pyruvatekinase); (iv) pgk (phosphoglycerate kinase); (v) gap2 (G3P-dehydrogenase); (vi) glgC (glucose-1-phosphateadenylyltransferase gene) involved in glycogen synthesis; (vii) pgm (phosphoglucomutase); (viii) pfkA (phosphofructokinaseI); (ix) fbpI (fructose1,6-biphosphataseI); (x) fbaA (fructose biphosphatealdolaseII); (xi) pdhABCD (pyruvate dehydrogenase); (xii) icd (isocitrate dehydrogenase).

Collectively, these data suggest that Cd downregulates citrate synthesis and conversion into 2-oxoglutarate that connects carbon and nitrogen assimilation pathways. This prediction was substantiated by the Cd-mediated downregulation of numerous genes operating in nitrogen metabolism (see below).

Very interestingly, we noticed that many of the carbon metabolism genes downregulated by Cd code for ATP-consuming enzymes such as cmpABCD, fbpI, pgk, pfkA, prk and pyk1. This negative regulation can be viewed as a part of a global ATP-sparing response to the Cd stress (See below).

Similarly to Cd, H2O2 downregulated many Ci acquisition genes (see Additional file 4 panel E): ccm, cmp and sbt, as well and numerous carbon metabolism genes: pgk, gpmB (slr1124 and slr1945), eno, fbpI, carA, carB, pdhA, pdhB, pdhC and pdhD. By contrast, Fe and Zn downregulated a few Ci acquisition genes such as cmp and sbt (Fe) and ccm (Zn), and had very little influence on carbon metabolism genes.

Cd and H2O2 downregulate nitrogen metabolism genes, many of which encode ATP-consuming enzymes

Most genes for the acquisition and assimilation of nitrogen were negatively regulated by both Cd and H2O2 (see Additional file 4 panel F), namely: (i) amt1 and amt2 (ATP-requiring ammonium permease); (ii) nrtABCD operon (ATP-requiring uptake of nitrate); (iii) urtABC (ATP-requiring uptake of urea); (iv) narB (nitrate reductase); (v) nirA (nitrite reductase); (vi) glnA and glnN (the two ATP-requiring glutamine synthase); (vii) murI (peptidoglycan synthesis); (viii) argB (ATP-dependent N-acetylglutamate kinase for arginine synthesis), (ix) cphA (ATP-requiring [48] cyanophycin synthetase); (x) hemA, hemF and hemL (synthesis of PS pigments, see Additional file 4 panel B). Consistent with the downregulation of the glutamine synthase glnA gene we found that both Cd and H2O2 upregulate the gifA and gifB genes, which code for an inhibitor of GlnA activity [49]. In addition a few related genes were specifically downregulated by either Cd (hemE and hemN), or H2O2. The latter were the following: (i) ureA and ureF (urease); (ii) carAB (ATP-requiring carbamoyl phosphate synthase); (iii) glsF (ferredoxin-dependent glutamate synthase); (iv) arG (ATP-requiring argininosuccinate synthase for arginine synthesis); (v) argH (argininosuccinate lyase); (vi) cphA (ATP-requiring [48] cyanophycin synthetase); and (vii): proA (ATP-dependent gamma-glutamyl phosphate reductase for proline synthesis).

Together, our data strongly show that Synechocystis challenged with H2O2 or Cd downregulates numerous key genes encoding ATP-consuming enzymes involved in nitrogen acquisition and metabolisms. This finding is consistent with the above-mentioned negative regulation of ATP-requiring mechanisms for carbon assimilation and metabolism, and global protein synthesis (See above). We view these downregulations as an ATP-sparing process aimed at compensating the decline in ATP production caused by the negative regulation of ATPase and photosynthesis genes.

Fe (but not Zn) regulated numerous N acquisition and assimilation genes, suggesting that Fe homeostasis and nitrogen assimilation are intrinsically connected.

Cd and H2O2 downregulate the two sulfur assimilation genes encoding ATP-dependent enzymes

Very interestingly, the genes met3 (sulfate adenylyltransferase) and cysC (adenylylsulfate kinase) encoding the two ATP-requiring enzymes of the cysteine-synthesis pathway appeared to be downregulated by both Cd and H2O2 (see Additional files 2, 3, 4). These data substantiate the Cd- and H2O2-elicited downregulation of ATP-consuming metabolic enzymes mentioned above.

Prominent role of the Slr1738 regulator in the transcriptional responses and survival to Cd

To demonstrate that the Cd-elicited breakdown of the photosynthetic (PS) machinery (Fig. 3A) is a direct physiological response rather than a side effect of cell damage, we searched for a regulator controlling this breakdown process with the view that its inactivation in interfering with the PS decline should decrease the level of tolerance to Cd. Hence, we became interested in the slr1738 transcription regulator gene because it is upregulated by Cd (Fig. 1 and see Additional file 4 panel C), a finding which suggests that Slr1738 might be involved in the responses to Cd. We have deleted the slr1738 gene (see Methods) and found the corresponding slr1738 null-mutant (Δslr1738) to be fully viable in standard growth conditions (Fig. 5), in agreement with the small number of genes with an altered level of expression (23, data not shown). As expected, the Δslr1738 mutant was found to be less resistant to Cd than the WT strain (Fig. 5B and 5D), indicating that Slr1738 mediates some of the Cd-elicited regulations. To characterize the Slr1738-mediated responses to Cd, we used DNA microarrays to identify the genes whose transcript abundance in Cd-treated cells differed at least twofold between the Δslr1738 mutant and the WT strain. As expected, we found that the Cd-elicited downregulation of PS genes (PSII large subunits, PBS, pigments synthesis, ATPases, cytochrome b6/f complex) and the simultaneous upregulation of the nblA genes operating in phycobilisomes (PBS) degradation were all impaired in the slr1738 null mutant (see Additional file 4 panel B). These findings were confirmed through absorption-spectroscopy analyses of the cellular content of PS proteins. As expected, the Cd-elicited decline of pigmented proteins was truly lower in the Δslr1738 mutant than in the WT strain (compare Fig. 3E with Fig. 3A). The Cd-elicited downregulation of the genes coding for ribosomal proteins was also impaired in the Δslr1738 mutant (see Additional file 4 panel B). Slr1738 was also found to contribute to the Cd-mediated upregulation of the suf genes involved in Fe-S cluster assembly and repair (see Additional file 4 panels C and D), and the ars genes operating in tolerance to arsenic and cadmium (Fig. 4).

thumbnailFigure 5. Influence of the slr1738 gene on the tolerance to cadmium and oxidative stress. Panel A: Typical survival to H2O2 of the wild type (WT, triangles) and Δslr1738 mutant (circles) cells. Panels B, C and D: typical growth of the WT and Δslr1738 cells cultivated in liquid (Panels B and C) or solid (Panel D) BG11 media with or without H2O2, paraquat and CdSO4 at the indicated concentration. These experiments were repeated three times.

By contrast, Slr1738 is likely not involved in the Cd-mediated regulation of carbon metabolism (see Additional file 4 panel E), indicating that other Cd response regulator(s) remain to be identified.

Finally, the Δslr1738 mutant appeared to be more resistant to H2O2 and paraquat than the WT strain (Fig. 5A and 5C). This phenotype, unnoticed by previous workers [50,51], is consistent with the increased level of expression of various antioxidant genes (see Additional file 4) such as the sll1621 peroxiredoxin gene [50-52] and the sequence homology between Slr1738 and the B. subtilis PerR (

egulator whose inactivation increases the resistance to oxidative stress [53].


Photosynthetic organisms that support much of the biosphere are increasingly challenged by heavy metals, which are persistent in the environment since they cannot be degraded. Using the model cyanobacterium Synechocystis PCC6803 as the host, we have performed a thorough multidisciplinary analysis of the global responses of photosynthetic cells to cadmium (Cd), as well as to hydrogen peroxide (H2O2) and noxious concentrations of the essential metals iron and zinc because the disturbance of metal homeostasis can generate oxidative stress [9]. The presently reported data on the global responses to both the Cd and Zn stresses are novel. In the case of the H2O2 and Fe stresses our data confirmed and extended those obtained previously after less-extensive investigations. In the case of the H2O2 stress, the novelty of our report is that our temporal analysis made it possible to discriminate between early and late responses, unlike the previously performed single time-point analysis [51]. Concerning the Fe-starvation stress, we choose to study the responses to a continuous Fe limitation because stresses occurring in Nature are durable, whereas Singh and co-workers studied cells recovering from a transient Fe deficiency [38]. This presumably explains the fact that we observed the induction of a larger number of Fe acquisition genes than the previous workers. Furthermore, unlike previous workers we also studied the response to Fe excess to identify those genes oppositely responding to excess and deficiency of Fe, which are therefore likely responding to Fe per se rather than to another indirect stress signal.

The presently reported occurrence of two temporal phases in the responses to Cd and H2O2, with a stress-specific timing, emphasizes on the value of kinetic analyses of stress responses especially when they are to be compared. Indeed, one cannot assume that two stresses of equal duration and toxicity have the same biological effects, in term of gene regulation. This important fact is illustrated by our findings that cells challenged with Cd (50 μM) or H2O2 (3 mM) for the same period of time (i.e. 30 min.) leading to equal lethality (2%) displayed massive responses to H2O2 but only moderate (early) responses to Cd in term of the number of the genes regulated (see Additional files 2, 3, 4).

The biological significance of the genome-wide transcriptional responses to all presently tested stresses (Table 1, and see Additional file 1, 2, 3, 4, 5, 6) was validated using relevant assays (see Figs. 1 to 5) performed with appropriate metal doses that varied with the number of cells to be treated, and/or attested by the following evidences. First, in every case all gene members of the same operon were found to be co-regulated. Second, all Fe- and Zn-acquisition genes responded the expected way to the availability of their cognate metal. Third, most of the dispersed genes encoding the protein-subunits of the same complex were found to be co-regulated, as observed for instance in the case of the photosynthesis (PS) genes (see Additional file 4 panel B). Fourth, in agreement with the Cd-mediated downregulation of most genes PS and the concomitant upregulation of protein-degradation genes (see Additional file 4 panel A), we showed that Cd decreases both the abundance (Fig. 3) and activity (See oxygen evolution data in the Results section) of the PS machinery. Fifth, consistent with the Cd-elicited induction of the arsenate reductase gene arsC (see Additional file 4 panel C) we showed that arsC operates in Cd tolerance (Fig. 4). Thus, the ArsC enzyme has a great biotechnological potential in contributing to the tolerance to two widespread persistent pollutants: arsenic and cadmium. Possibly the ArsC enzyme, which employed glutathione (GSH) and glutaredoxin as reductants [45], could somehow "sequester" Cd in generating an hypothetical GSH-Cd complex less toxic than Cd. Sixth, as inferred from the Cd-elicited upregulation of numerous high light-inducible genes (see Additional file 4 panel A) we showed that Cd decreases the tolerance to light (Fig. 2C). Seventh, as anticipated from its Cd-elicited induction (Fig. 1 and see Additional file 4 panel C) we demonstrated that the Slr1738 regulator mediates several responses that are crucial to protection against Cd, such as the decline of the PS machinery and the upregulation of the arsC gene (Fig. 3, Fig. 4 and Fig. 5 and see Additional file 4).

Additional file 5. Dye-Swap-correlations. The data provided indicate (for each time of the 5 kinetics) the correlation coefficient between the two ratio samples obtained with the two microarrays of each dye-swap.

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Additional file 6. GSM numbers of all microarray experiments. This table provides the links for accessing our microarray data which have been deposited in the GEO website.

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The occurrence of large clusters of co-regulated genes, encoding ribosome, ATPase or Fe uptake proteins, suggests a mechanism of global control of gene expression involving chromosomal structure, similarly to chromatin remodeling in eukaryotic cells. This prediction is comforted by the findings (see Additional file 2) that the Synechocystis HU and Dps nucleoprotein genes, possibly involved in such structure-dependent global regulation [54], are regulated by Cd (see Additional file 4), positively (HU, sll1712) or negatively (Dps, slr1894).

Many of our data support the notion of metal selectivity. For examples, (i) Fe but not Zn mimicked the Cd-mediated downregulation of N acquisition and assimilation genes (see Additional file 4 panel F); and (ii) Zn but not Fe mimicked the Cd-mediated decline of the PS machinery (Fig. 3 and see Additional file 4) and the downregulation of ribosomal genes which is therefore not a general stress response. By contrast, numerous genes responded the same way to Cd, Fe, Zn and H2O2 (see Additional file 4), suggesting that reactive oxygen species might act in signal transduction of stress responses, as proposed [55].

Both H2O2 and Cd were found to upregulate numerous genes operating in tolerance to oxidative stress (see Additional file 4 panel D) and to the related high light stress (see Additional file 4 panel B) that also generates toxic

pecies (ROS) [23]. These findings, which were anticipated in the case of the H2O2 stress, were confirmed by showing that both H2O2 and Cd render cells light sensitive (Fig. 2C). In addition, both H2O2 and Cd upregulated the suf genes (see Additional file 4 panel D) involved in iron-sulfur cluster biogenesis or repair [39] and the Fe uptake genes (see Additional file 4 panel C; all genes in the case of H2O2 and half of them in the case of Cd). Consistently, we found that increasing the concentration of Fe in the medium increases the cell tolerance to both H2O2 and Cd (Fig. 2). These results are reminiscent to what occurs in oxidative-stressed E. coli cells [40-42]. They suggest that Synechocystis challenged with either H2O2 or Cd accelerates Fe uptake strongly (H2O2) or moderately (Cd) to provide extra Fe atoms for the repair of damaged Fe-S clusters. Having also noticed that Cd triggers a larger breakdown of the Fe-rich PS machinery than H2O2 (Fig. 3), we believe that cells challenged with H2O2 or Cd use two strategies to provide extra Fe atoms to the machinery that synthesizes or repairs Fe-S centers. H2O2-treated cells undergoing a limited PS-decline (Fig. 3D) mostly accelerate the intake of Fe from the medium, while Cd-stressed cells that moderately increase Fe intake breakdown a part of their abundant PS machinery (Fig. 3A), which normally contains 21–23 Fe atoms per PS unit [43]. Consequently, we predicted, and confirmed, that increasing the availability of Fe limits the Cd-elicited decline of the PS-machinery (Fig. 3).

Many of the key genes involved in acquisition and metabolism of C, N and S that were downregulated by Cd and H2O2 are coding for ATP-consuming enzymes (see Additional file 4 panels E and F). These responses can be viewed as an ATP-sparing process used by the cells to compensate for the decreased production of ATP caused by the decline of the PS apparatus (Cd and to a lesser extent H2O2) or of the respiration machinery (H2O2 but not Cd downregulates cytochrome oxidase genes, See TableS4B). Similarly, the downregulation of ribosomal genes (see Additional file 4 panel A) encoding normally abundant proteins [22] triggered by cells facing Cd and H2O2 is likely aimed at sparing C, N and S nutrients to compensate for the downregulation of the corresponding acquisition and assimilation genes.

Based on the presently reported findings, we view the responses to a continuous Cd stress as a two temporal-phases process. In the early phase occurring during the first 60 min of exposure (Table 1), Cd-stressed cells regulate mainly the genes operating in metal transport (see Additional file 4 panel C) and protein maturation and degradation (see Additional file 4 panel A). These responses presumably limit Cd entry into the cells and incorporation in place of the cognate metal cofactor of metalloproteins. In prolonged exposures (after 60 min.), these regulations are conserved, and even amplified in term of the number of responsive processes, thereby defining the next phase designated as "massive" for this reason. At this stage, the responses to Cd can be viewed as an integrated "Yin Yang" reprogramming of the whole cellular metabolism. As the Yin process, most key genes operating in uptake and assimilation of inorganic nutrients (C, N and S) and protein synthesis are turned down. These responses allow (i) the sparing of both energy (ATP) and reducing power (NAD(P)H) normally consumed by nutrient assimilation and subsequent metabolism, and (ii) the limitation of the poisoning incorporation of Cd in metalloenzymes. As the compensatory Yang process, the PS breakage of the PS machinery, which decreases the production of both ATP and NADPH, liberates Fe and C, N and S nutrient assimilates that can be recycled into the synthesis of Cd-tolerance enzymes such as the ArsC arsenate reductase (Fig. 4) and, presumably, other Cd-inducible enzymes: Suf proteins (see above), flavodoxin (IsiB), ferredoxin (FedII), flavoproteins (Flv2 and Flv4), glutathione peroxidase (Gpx1), peroxiredoxin (Ahpc-like), thioredoxin (TrxA), hydrogenase subunits (HypA, D, E) and the ZiaA (the ATPase homologous to the cadmium export ATPase of other organisms). Furthermore, other new Cd-tolerance enzymes might be discovered in the future, among the product of the orphan genes which apperaed to be upregulated by Cd (see Additional files 1 and 3). We showed that the Cd-induced Slr1738 regulator (Fig. 1 and TableS4) plays a central role in the protection against Cd (Fig. 5) in mediating several of the important regulations, such as the breakage of the PS machinery, the downregulation of ribosomal genes, and the upregulation of the arsC arsenate reductase and suf genes.


Using the cyanobacterium Synechocystis PCC6803 as a model organism, we analyzed the global responses of environmentally important cells to stresses triggered by Cd (an abundant persistant pollutant), H2O2 (the paradigm ROS agent), or drastic changes in Fe availability, which appeared to modulate the tolerance to Cd and H2O2. Our results indicate that cells challenged with H2O2 or Cd use different strategies for the same purpose of increasing the supply of Fe atoms to the synthesis and repair of Fe-requiring metalloenzymes. While H2O2-challenged cells preferentially accelerate Fe intake, Cd-stressed cells preferentially breakdown the Fe-rich PS machinery to liberate Fe atoms. We view the responses to Cd as an integrated "Yin Yang" metabolic reprogramming. As the "Yin" process, the ATP- and nutrients-sparing downregulation of anabolism limits the poisoning incorporation of Cd into metalloenzymes. As the compensatory "Yang" process, the PS breakdown liberates nutrient assimilates for the synthesis of Cd-tolerance proteins. We found that this reprogramming is mediated by the Slr1738 transcriptional regulator that also operates in oxidative stress tolerance, in agreement with its sequence homology with the peroxide resistance regulator of Bacillus subtilis. Further studies will be necessary to understand the influence of Cd and H2O2 on the activity of Slr1738.


Bacterial strains, growth and survival analyses

The unicellular cyanobacterium Synechocystis PCC6803 was grown as described [56] at 30°C in liquid BG11 medium enriched with Na2CO3 (3.78 mM, final concentration), under continuous white light of standard fluence (2,500 luxes, i.e. 31.25 μE.m-2.s-1). When required kanamycin 50–300 μ was added to the cultures. For growth assays, cells grown three times up to mid log phase (OD580 0.5 units, i.e. 2.5 × 107 were inoculated into fresh BG11 medium with or without CdSO4 or paraquat (at the indicated concentration), and OD580 were measured at time intervals. For survival analysis, cells in mid log phase were incubated for 1 h with H2O2 (at the indicated concentration), washed twice with BG11, plated on solid BG11, and the colonies were counted after 5–7 days of incubation under standard conditions. The influence of various agents on the growth and survival on solid media was assayed as follows. Four fold serial dilutions of liquid cultures were spotted as 15 μl dots onto BG11 plates with or without the indicated concentration of the tested agents. Plates were then incubated for 4–5 days under standard conditions prior to scanning. For survival analysis, cells were harvested, washed and resuspended in BG11 medium prior to plating and counting.

Construction of knockout mutants of Slr0946 (arsenate reductase) and Slr1738 regulator

Specific oligonucleotides were used for PCR amplification from the Synechocystis genome [57] of each studied gene along with its two 0.3 kb-long flanking DNA segments that serve as platforms for homologous recombinations mediating targeted gene replacement [58]. The PCR products were independently cloned in the pGEMt plasmid (Pharmacia), and inactivated as follows. For slr0946, an internal 223 bp segment (starting from the 7th nucleotide downstream of its GTG start codon) was substituted by the StuI restriction site that was subsequently used to insert the HincII Kmr cassette originating from the pUC4K plasmid (Pharmacia). For slr1738 inactivation, the 388 bp segment beginning 6 nucleotides behind the ATG start codon was replaced by the SmaI site in which we cloned the HincII Kmr cassette. The resulting deletion cassettes slr0946::Kmr and slr1738::Kmr were sequenced (Big Dye kit, ABI Perking Elmer) prior to transformation to Synechocystis. Through PCR and sequence analyses, we verified that the antibiotic resistant marker had been inserted properly in the genome of the transformant clones, i.e. in place of the corresponding studied gene. These nullmutants grew healthy in standard conditions, demonstrating that both Slr0946 and Slr1738 proteins are dispensable to the viability of Synechocystis.

Photosynthetic pigments determination by absorption spectrometry

Absorption spectra of whole cells grown or challenged on solid medium and resuspended in water, were monitored with a DU640 spectrophotometer (Beckman). Samples were adjusted for equal scattering at 800 nm. Carotenoids absorb light between 350 and 540 nm. The absorption maximum for phycocyanin is 630 nm and that for chlorophyll a are 442 nm and 681 nm. These experiments were repeated at least three times.

RNA isolation

Because of their short half lives typical of prokaryotic transcripts, Synechocystis mRNA were rapidly prepared (in less than 2 min.) from cells grown or challenged on solid media as described [56]. Briefly, 300 ml of mid-log phase liquid cultures (2.5 × grown in standard conditions were rapidly concentrated 40-fold by centrifugation and spotted as 20 μl dots on BG11 plates with, or without (control samples), CdSO4 (50 μM); (NH4)FeH2C6H5O7 (17 μM); ZnSO4 (776 μM); or H2O2 (3 mM). Then, plates were incubated for the indicated times (in min) prior to cell harvest and fast disruption with an Eaton press. Iron depletion analyses were performed with liquid cell suspensions to avoid uncontrolled liberation of Fe atoms from agar. Hence, exponentially growing cells washed in Fe-free medium were challenged for 48 h in liquid medium containing 0 to 2 μM of (NH4)FeH2C6H5O7. Then, cells were harvested by centrifugation and resuspension and disrupted. RNA were extracted [56] with the RNeasy kit from Qiagen (DNA microarrays kit) and treated with RNase-free DNase I (Roche). The RNA concentration and purity were determined by A260 and A280 measurement (A260/A280 > 1.9), as well as by migration on agarose gel to verify the absence of RNA degradation.

DNA-microarray data acquisition and statistical analysis

The microarrays data presently reported have been deposited in the MIAME compliant NCBIs Gene Expression Omnibus [59] under the accession number GSE3755 (see Additional file 6) DNA microarrays (IntelliGene™ CyanoCHIP version 1.2 or 2.0, Takara), covering 2,891 (CyanoCHIP1.2) or 2,954 (CyanoCHIP2.0) of the 3,168 ORFs of Synechocystis were purchased from Cambrex Bio Science and manipulated as described [56]. Test RNA (from stressed cells) and corresponding control RNA (untreated cultures) were reverse transcribed, differentially labeled with Cy3 and Cy5 dyes and hybridized in a replicate dye swap. Arrays were immediately scanned with a GenePix™ 4000B scanner (Axon Instruments), and images were analyzed with GenePixTM Pro 4.0 (Molecular Devices). Spots were considered when they lack blemishes, deformations or dusts, and their fluorescence signal exceeded the local background plus 2 standard deviations. Then, signal intensity was determined by subtracting local background of each spot (GenePix™ Pro 4.0). Each GenePix Result file (.GPR) was converted to a TIGR Array viewer file (.TAV) using TIGR ExpressConverter version 1.7 for signal analysis. All spot intensities have been normalized with the LOWESS method [60], using the locfit function of the TIGR Midas version 2.19 [61] with the smooth parameter set to 0.33 as recommended [62]. Normalized measures served to compute the ratios of Cy3/Cy5 intensity and the associated log2-transform (denote log2-ratios) for each gene. For each replicated dye-swap, the average expression ratio of a given gene is calculated as the geometric mean of the two ratios [62].

Three lines of evidences attested the quality of our data. First our normalization method was validated with both internal (positive: Synechocystis DNA; negative: Salmon sperm DNA) and external (human TFR mRNA) controls. Second, for each dye-swap, correlation coefficients calculated between both replicates (see Additional file 5) appeared to be greater than 0.9 in most cases, thereby attesting the within-study reproducibility. Third, to analyze the within-platform variations, Cy3- and Cy5-labeled cDNAs were prepared from a single preparation of total RNA from unstressed cells, mixed together and hybridized to a microarray. The distribution of expression ratio ((see Additional files 4 and 6) showed that 90% of them fall within the range (0.80 – 1.25) and 99% within the range (0.64 – 1.57). Moreover, the mean of this distribution is equal to 1.00 (standard deviation equal to 0.13). Consequently, we felt confident to regard as regulated any particular gene the expression level of which was changed at least 1.9 fold.

Identification of the two temporal phases of the responses to Cd and H2O2

We have considered each cDNA array as a split replicate, without averaging the dye-swap values. For each stress, the microarray data were dispatched in two groups each corresponding to a presumed kinetic phase. In the case of Cd the first group of data (15 mins to 60 mins) contains 8 replicates and the second (90 min to 360 min) contains 10 replicates, while for H2O2 the first group (15 min to 30 min) contains 4 replicates and the second (180 min to 420 min) contains 6 replicates. To identify genes with log2-ratios significantly different between the two time phases, p-values were first calculated for each gene using a moderated t-test based on an empirical Bayes analysis that is equivalent to shrinkage (or expansion) of the estimated sample variances towards a pooled estimate, resulting in a more stable inference. The p-values of the t-test were adjusted for multiple hypotheses testing, controlling the false discovery rate (FDR) as proposed by [63]. Thus, using a cut-off of the adjusted p-values at 0.05 gives and approximate level of False Discovery Rate (FDR) at 0.05. Using a strict cut-off of p = 1e-3 we found 791 genes differentially expressed in the two kinetic phases of responses to Cd (see Additional file 2) and 228 phase-responsive genes in the case of H2O2 (TableS3). The statistical analysis was carried out in the R language release 2.2, using the package limma [64] from the Bioconductor project [65].

Measurement of photosynthetic activity

Cells incubated for 3 and 6 h on solid BG11 medium with or without CdSO4 (50 μM) were washed and resuspended in BG11 medium as described in the RNA isolation section. Photosynthetic oxygen-evolving activity of intact cells was measured at 30°C under saturating light intensity with a Clark-type oxygen electrode (Hansatech).

Over-expression and purification of the Slr1738 protein fused to a hexahistidine tag

The Slr1738 coding sequence was PCR amplified from the Synechocystis genome, using appropriate oligonucleotide primers to embed its ATG initiation codon into a Nde I restriction site and introduce a Bam HI site behind its stop codon. The resulting Nde I-Bam HI restriction fragment was cloned into the pET28 (+) E. coli expression vector opened with the same enzymes, thereby allowing the in-frame fusion of the 6 × His tag with the Slr1738 amino acids sequence. After sequence verification (Big Dye kit, ABI Perking Elmer) the pET28-1738 plasmid was transformed into E. coli BL21 (DE3) selecting for resistance to kanamycin (50 μ Transformant cells were grown at 37°C in Km-containing Luria Bertani medium up to an optical density (A600) of 0.8. At that time, 1 mM isopropyl-thio-β-D-galactopyranoside (IPTG) was added to induce the synthesis of the 6 × His-Slr1738 protein, and cells were further incubated for 15 h at 30°C, harvested by centrifugation and resuspended in 20 ml of 20 mM Tris pH 8.0, 500 mM NaCl and 5 mM imidazole (lysis buffer). Cells were disrupted by sonication (Microson), centrifuged at 14,000 g for 20 min at 4°C, and the supernatant was applied to a nickel-nitrilotriacetic acid-agarose column (3 ml) equilibrated with 25 ml of lysis buffer. After washings with 30 ml of lysis buffer and buffer A (20 mM Tris pH 8.0, 500 mM NaCl and 50 mM imidazole), recombinant proteins were eluted with 6 ml of buffer B (20 mM Tris pH 8.0, 500 mM NaCl and 500 mM imidazole). 6His-Slr1738 containing fractions were pooled, desalted on a PD10 Sephadex G-25M column (Amersham Biosciences). The Purity of the 6His-Slr1738 protein was greater than 95%, as judged by SDS-PAGE electrophoresis.

Western blot analysis of selected proteins

Crude cell extract (5 μg) of Synechocystis cells incubated on solid media with or without CdSO4 (50 μM, 360 min.) or H2O2 (3 mM, 30 min.) were harvested, disrupted (see above), electrophoresed on 13% SDS-PAGE [66] and transferred onto nitrocellulose sheets as described [67]. For detections we use the following rabbit antibodies: anti-Slr1738 (this work, dilution 1:20000); anti-psaC (dilution 1:1000) or anti-rbcL (dilution 1:5000) from Agrisera; anti-IsiA (kindly provided by Dr. A. Wilde, dilution 1:5000); anti-IsiB (kindly provided by Dr. M. Hagemann, dilution 1:5000). Horseradish peroxidase-conjugated goat anti-rabbit antibodies (dilution 1:4000) were used as second antibody, and immune complexes were revealed by chemiluminescence (ECL kit, Amersham Biosciences).

Authors' contributions

LH participated in the design and realization of the transcriptome experiments in the wild type strain. MF constructed the slr1738 null mutant and carried out the transcriptome and phentotypic analysis of this strain. BM carried out the construction and phenotypic analyses of the arsC-null mutant, and participated to other cell-fitness analyses. MM participated in the bioinformatics analysis of the transcriptome data, and in the drafting of the relevant part of the manuscript. AP carried out the overproduction of Slr1738 protein and corresponding antibodies and performed the Western blot experiments. PL participated in the analysis and interpretation of the transcriptome data and in the drafting of the whole manuscript. JCA participated in the bioinformatics analysis of the transcriptome data, and in the drafting of the relevant part of the manuscript. CCC participated in the conception, acquisition, analysis and interpretation of all data; carried out the oxygen evolution measurements; and participated in the drafting of the whole manuscript. FC participated in conception and supervision of the whole study, and wrotes the manuscript. All authors read and approved the final manuscript.


This work was supported by the French scientific Programs "Toxicologie Nucléaire Environnementale" and "ANR Biosys06_134823: SULFIRHOM". L.H, B.M, M.M and A.P were recipients of fellowships from the CEA (France). M.F. was recipient of MENESR PhD fellowship. We thank A. Wilde and M. Hagemann for their kind gift of antibodies directed against the IsiA and IsiB proteins, respectively; and C. Creminon and J-C. Robillard for their help in the preparation of antibodies directed against Slr1738.


  1. Partensky F, Hess WR, Vaulot D: Prochlorococcus, a marine photosynthetic prokaryote of global significance.

    Microbiol Mol Biol Rev 1999, 63:106-127. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Zehr JP, Waterbury JB, Turner PJ, Montoya JP, Omoregie E, Steward GF, Hansen A, Karl DM: Unicellular cyanobacteria fix N2 in the subtropical North Pacific Ocean.

    Nature 2001, 412:635-638. PubMed Abstract | Publisher Full Text OpenURL

  3. Nishiyama Y, Yamamoto H, Allakhverdiev SI, Inaba M, Yokota A, Murata N: Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery.

    EMBO J 2001, 20(20):5587-5594. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  4. Satarug S, Baker JR, Urbenjapol S, Haswell-Elkins M, Reilly PE, Williams DJ, Moore MR: A global perspective on cadmium pollution and toxicity in non-occupationally exposed population.

    Toxicol Lett 2003, 137(1-2):65-83. PubMed Abstract | Publisher Full Text OpenURL

  5. Andrew AS, Warren AJ, Barchowsky A, Temple KA, Klei L, Soucy NV, O'Hara KA, Hamilton JW: Genomic and proteomic profiling of responses to toxic metals in human lung cells.

    Environ Health Perspect 2003, 111(6):825-835. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  6. Waisberg M, Joseph P, Hale B, Beyersmann D: Molecular and cellular mechanisms of cadmium carcinogenesis.

    Toxicology 2003, 192(2-3):95-117. PubMed Abstract | Publisher Full Text OpenURL

  7. Rosenzweig AC: Metallochaperones: bind and deliver.

    Chem Biol 2002, 9(6):673-677. PubMed Abstract | Publisher Full Text OpenURL

  8. Bryant DA: The molecular biology of cyanobacteria. In Advances in photosynthesis. Volume 1. Edited by Govindjee . Dordrecht , Kluwer academic publishers; 1994. OpenURL

  9. Stohs SJ, Bagchi D: Oxidative mechanisms in the toxicity of metal ions.

    Free Radic Biol Med 1995, 18(2):321-336. PubMed Abstract | Publisher Full Text OpenURL

  10. Ferris MJ, Palenik B: Niche adaptation in ocean cyanobacteria.

    Nature 1998, 396:226-228. Publisher Full Text OpenURL

  11. Peschek GA: Structure-function relationships in the dual-function photosynthetic-respiratory electron-transport assembly of cyanobacteria (blue-green algae).

    Biochem Soc Trans 1996, 24(3):729-733. PubMed Abstract OpenURL

  12. Martin W, Rujan T, Richly E, Hansen A, Cornelsen S, Lins T, Leister D, Stoebe B, Hasegawa M, Penny D: Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus.

    Proc Natl Acad Sci U S A 2002, 99:12246-12251. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  13. Gray MW: Origin and evolution of organelle genomes.

    Curr Opin Genet Dev 1993, 3(6):884-890. PubMed Abstract | Publisher Full Text OpenURL

  14. Drazkiewicz M, Tukendorf A, Baszynski T: Age-dependent response of maize leaf segments to cadmium treatment: effect on chlorophyll fluorescence and phytochelatin accumulation.

    J Plant Physiol 2003, 160(3):247-254. PubMed Abstract | Publisher Full Text OpenURL

  15. Carrier P, Baryla A, Havaux M: Cadmium distribution and microlocalization in oilseed rape (Brassica napus) after long-term growth on cadmium-contaminated soil.

    Planta 2003, 216(6):939-950. PubMed Abstract | Publisher Full Text OpenURL

  16. Bachmann T: Transforming cyanobacteria into bioreporters of biological relevance.

    Trends Biotechnol 2003, 21(6):247-249. PubMed Abstract | Publisher Full Text OpenURL

  17. Gong R, Ding Y, Liu H, Chen Q, Liu Z: Lead biosorption and desorption by intact and pretreated spirulina maxima biomass.

    Chemosphere 2005, 58(1):125-130. PubMed Abstract | Publisher Full Text OpenURL

  18. Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S: Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.

    DNA Res 1996, 3:109-136. PubMed Abstract | Publisher Full Text OpenURL

  19. Mazouni K, Bulteau S, Cassier-Chauvat C, Chauvat F: Promoter element spacing controls basal expression and light-inducibility of the cyanobacterial secA gene.

    Mol Microbiol 1998, 30:1113-1122. PubMed Abstract | Publisher Full Text OpenURL

  20. Poncelet M, Cassier-Chauvat C, Leschelle X, Bottin H, Chauvat F: Targeted deletion and mutational analysis of the essential (2Fe-2S) plant-like ferredoxin in Synechocystis PCC6803 by plasmid shuffling.

    Mol Microbiol 1998, 28:813-821. PubMed Abstract | Publisher Full Text OpenURL

  21. Mazouni K, Domain F, Cassier-Chauvat C, Chauvat F: Molecular analysis of the key cytokinetic components of cyanobacteria: FtsZ, ZipN and MinCDE.

    Mol Microbiol 2004, 52(4):1145-1158. PubMed Abstract | Publisher Full Text OpenURL

  22. Mrazek J, Bhaya D, Grossman AR, Karlin S: Highly expressed and alien genes of the Synechocystis genome.

    Nucleic Acids Res 2001, 29(7):1590-1601. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  23. Munekage Y, Hashimoto M, Miyake C, Tomizawa K, Endo T, Tasaka M, Shikanai T: Cyclic electron flow around photosystem I is essential for photosynthesis.

    Nature 2004, 429(6991):579-582. PubMed Abstract | Publisher Full Text OpenURL

  24. Cavet JS, Borrelly GP, Robinson NJ: Zn, Cu and Co in cyanobacteria: selective control of metal availability.

    FEMS Microbiol Rev 2003, 27(2-3):165-181. PubMed Abstract | Publisher Full Text OpenURL

  25. van Waasbergen LG, Dolganov N, Grossman AR: nblS, a gene involved in controlling photosynthesis-related gene expression during high light and nutrient stress in Synechococcus elongatus PCC 7942.

    J Bacteriol 2002, 184(9):2481-2490. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  26. Hihara Y, Kamei A, Kanehisa M, Kaplan A, Ikeuchi M: DNA microarray analysis of cyanobacterial gene expression during acclimation to high light.

    Plant Cell 2001, 13(4):793-806. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  27. Huang L, McCluskey MP, Ni H, LaRossa RA: Global Gene Expression Profiles of the Cyanobacterium Synechocystis sp. Strain PCC 6803 in Response to Irradiation with UV-B and White Light.

    J Bacteriol 2002, 184(24):6845-6858. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  28. Tu CJ, Shrager J, Burnap RL, Postier BL, Grossman AR: Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803.

    J Bacteriol 2004, 186(12):3889-3902. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  29. He Q, Dolganov N, Bjorkman O, Grossman AR: The high light-inducible polypeptides in Synechocystis PCC6803. Expression and function in high light.

    J Biol Chem 2001, 276(1):306-314. PubMed Abstract | Publisher Full Text OpenURL

  30. Yeremenko N, Kouril R, Ihalainen JA, D'Haene S, van Oosterwijk N, Andrizhiyevskaya EG, Keegstra W, Dekker HL, Hagemann M, Boekema EJ, Matthijs HC, Dekker JP: Supramolecular organization and dual function of the IsiA chlorophyll-binding protein in cyanobacteria.

    Biochemistry 2004, 43(32):10308-10313. PubMed Abstract | Publisher Full Text OpenURL

  31. Silva P, Thompson E, Bailey S, Kruse O, Mullineaux CW, Robinson C, Mann NH, Nixon PJ: FtsH is involved in the early stages of repair of photosystem II in Synechocystis sp PCC 6803.

    Plant Cell 2003, 15(9):2152-2164. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  32. Schneider D, Berry S, Volkmer T, Seidler A, Rogner M: PetC1 is the major Rieske iron-sulfur protein in the cytochrome b6f complex of Synechocystis sp. PCC 6803.

    J Biol Chem 2004, 279(38):39383-39388. PubMed Abstract | Publisher Full Text OpenURL

  33. Thelwell C, Robinson NJ, Turner-Cavet JS: An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter.

    Proc Natl Acad Sci U S A 1998, 95(18):10728-10733. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  34. Garcia-Dominguez M, Lopez-Maury L, Florencio FJ, Reyes JC: A gene cluster involved in metal homeostasis in the cyanobacterium Synechocystis sp. strain PCC 6803.

    J Bacteriol 2000, 182(6):1507-1514. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  35. Rensing C, Ghosh M, Rosen BP: Families of soft-metal-ion-transporting ATPases.

    J Bacteriol 1999, 181(19):5891-5897. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  36. Raux E, Lanois A, Warren MJ, Rambach A, Thermes C: Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon.

    Biochem J 1998, 335 ( Pt 1):159-166. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  37. Katoh H, Hagino N, Grossman AR, Ogawa T: Genes essential to iron transport in the cyanobacterium Synechocystis sp. strain PCC 6803.

    J Bacteriol 2001, 183(9):2779-2784. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  38. Singh AK, McIntyre LM, Sherman LA: Microarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp. PCC 6803.

    Plant Physiol 2003, 132(4):1825-1839. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  39. Wang T, Shen G, Balasubramanian R, McIntosh L, Bryant DA, Golbeck JH: The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria.

    J Bacteriol 2004, 186(4):956-967. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  40. Benov L, Fridovich I: Growth in iron-enriched medium partially compensates Escherichia coli for the lack of manganese and iron superoxide dismutase.

    J Biol Chem 1998, 273(17):10313-10316. PubMed Abstract | Publisher Full Text OpenURL

  41. Zheng M, Wang X, Templeton LJ, Smulski DR, LaRossa RA, Storz G: DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide.

    J Bacteriol 2001, 183(15):4562-4570. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  42. Djaman O, Outten FW, Imlay JA: Repair of oxidized iron-sulfur clusters in Escherichia coli.

    J Biol Chem 2004, 279(43):44590-44599. PubMed Abstract | Publisher Full Text OpenURL

  43. Straus NA: Iron deprivation: Physiology and Gene Regulation. In The Molecular Biology of Cyanobacteria. Edited by Bryant DA. Dordrecht , Kluwer Academic Publisher; 1994:731-750. OpenURL

  44. Lopez-Maury L, Florencio FJ, Reyes JC: Arsenic sensing and resistance system in the cyanobacterium Synechocystis sp. strain PCC 6803.

    J Bacteriol 2003, 185(18):5363-5371. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  45. Li R, Haile JD, Kennelly PJ: An arsenate reductase from Synechocystis sp. strain PCC 6803 exhibits a novel combination of catalytic characteristics.

    J Bacteriol 2003, 185(23):6780-6789. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  46. Badger MR, Price GD: CO2 concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution.

    J Exp Bot 2003, 54(383):609-622. PubMed Abstract | Publisher Full Text OpenURL

  47. Figge RM, Cassier-Chauvat C, Chauvat F, Cerff R: Characterization and analysis of an NAD(P)H dehydrogenase transcriptional regulator critical for the survival of cyanobacteria facing inorganic carbon starvation and osmotic stress.

    Mol Microbiol 2001, 39:455-469. PubMed Abstract | Publisher Full Text OpenURL

  48. Aboulmagd E, Oppermann-Sanio FB, Steinbuchel A: Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity.

    Appl Environ Microbiol 2001, 67(5):2176-2182. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  49. Garcia-Dominguez M, Reyes JC, Florencio FJ: NtcA represses transcription of gifA and gifB, genes that encode inhibitors of glutamine synthetase type I from Synechocystis sp. PCC 6803.

    Mol Microbiol 2000, 35(5):1192-1201. PubMed Abstract | Publisher Full Text OpenURL

  50. Kobayashi M, Ishizuka T, Katayama M, Kanehisa M, Bhattacharyya-Pakrasi M, Pakrasi HB, Ikeuchi M: Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 6803.

    Plant Cell Physiol 2004, 45(3):290-299. PubMed Abstract | Publisher Full Text OpenURL

  51. Li H, Singh AK, McIntyre LM, Sherman LA: Differential gene expression in response to hydrogen peroxide and the putative PerR regulon of Synechocystis sp. strain PCC 6803.

    J Bacteriol 2004, 186(11):3331-3345. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  52. Hosoya-Matsuda N, Motohashi K, Yoshimura H, Nozaki A, Inoue K, Ohmori M, Hisabori T: Anti-oxidative stress system in cyanobacteria. Significance of type II peroxiredoxin and the role of 1-Cys peroxiredoxin in Synechocystis sp. strain PCC 6803.

    J Biol Chem 2005, 280(1):840-846. PubMed Abstract | Publisher Full Text OpenURL

  53. Bsat N, Herbig A, Casillas-Martinez L, Setlow P, Helmann JD: Bacillus subtilis contains multiple Fur homologues: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors.

    Mol Microbiol 1998, 29(1):189-198. PubMed Abstract | Publisher Full Text OpenURL

  54. Dorman CJ, Deighan P: Regulation of gene expression by histone-like proteins in bacteria.

    Curr Opin Genet Dev 2003, 13(2):179-184. PubMed Abstract | Publisher Full Text OpenURL

  55. Apel K, Hirt H: Reactive oxygen species: metabolism, oxidative stress, and signal transduction.

    Annu Rev Plant Biol 2004, 55:373-399. PubMed Abstract | Publisher Full Text OpenURL

  56. Domain F, Houot L, Chauvat F, Cassier-Chauvat C: Function and regulation of the cyanobacterial genes lexA, recA and ruvB: LexA is critical to the survival of cells facing inorganic carbon starvation.

    Mol Microbiol 2004, 53(1):65-80. PubMed Abstract | Publisher Full Text OpenURL

  57. Nakamura Y, Kaneko T, Hirosawa M, Miyajima N, Tabata S: CyanoBase, a www database containing the complete nucleotide sequence of the genome of Synechocystis sp. strain PCC6803.

    Nucl Acids Res 1998, 26:63-67. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  58. Labarre J, Chauvat F, Thuriaux P: Insertional mutagenesis by random cloning of antibiotic resistance genes into the genome of the cyanobacterium Synechocystis PCC6803.

    J Bacteriol 1989, 171:3449-3457. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  59. Edgar R, Domrachev M, Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository.

    Nucleic Acids Res 2002, 30(1):207-210. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  60. Cleveland WS: Robust locally weighted regression and smoothing scatterplots.

    J Amer Stat Assoc 1979, 74:829-836. Publisher Full Text OpenURL

  61. Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J: TM4: a free, open-source system for microarray data management and analysis.

    Biotechniques 2003, 34(2):374-378. PubMed Abstract OpenURL

  62. Quackenbush J: Microarray data normalization and transformation.

    Nat Genet 2002, 32 Suppl:496-501. PubMed Abstract | Publisher Full Text OpenURL

  63. Benjamini Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing.

    Journal of the Royal Statistical Society 1995, 57:289-300. OpenURL

  64. Gordon KS: Limma: linear models for microarray data. In Bioinformatics and Computational Biology Solutions using R and Bioconductor. Edited by Gentleman R, Carey V, Dudoit S, Irizarry R, Huber W. New York , Springer; 2005:397-420. OpenURL

  65. Gentleman R, Carey VJ, Bates DM, Bolstad BM, Dettlings M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J: Bioconductor: open software development for computational biology and bioinformatics. [ ] webcite

    Genome Biology 2004., 580 OpenURL

  66. Chua NH: Electrophoresis analysis of chloroplast proteins. Volume 69. Academic Press, INC.; 1980::434-436. [Methods in Enzymology]

  67. Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

    Proc Natl Acad Sci U S A 1979, 76(9):4350-4354. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL