ChIP on SNP-chip for genome-wide analysis of human histone H4 hyperacetylation
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* Corresponding author: Michael A Rudnicki mrudnicki@ohri.ca
1 Ottawa Health Research Institute, Regenerative Medicine Program, 501 Smyth Road, Ottawa, Ontario, K1H 8L6, Canada
2 Faculty of Health Sciences, McMaster University Hamilton, Ontario, L8S 4K1, Canada
BMC Genomics 2007, 8:322 doi:10.1186/1471-2164-8-322
Published: 14 September 2007Additional files
Additional file 1:
Supplementary Table S1. Genomic correlation of histone H4 hyperacetylation. For all pairs of SNP-array probes detecting genomic sites separated a given distance range, the table shows the numbers of pairs according to their detection status. Columns are: distance, upper limit of distance range (in bp); total_blast, number of pairs at the distance range for the myoblast sample; aa_blast, fraction of pairs where both probes did not detect histone H4 hyperacetylation in the myoblast sample; pp_blast, fraction of pairs where both probes detected histone H4 hyperacetylation in the myoblast sample; total_tube, number of pairs at the distance range for the myotube sample; aa_tube, fraction of pairs where both probes did not detect histone H4 hyperacetylation in the myotube sample; pp_tube, fraction of pairs where both probes detected histone H4 hyperacetylation in the myotube sample. This data was used for the graphs in Figure 2A.
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Additional file 2:
Supplementary Table S2. Genomic correlation gene expression. For all pairs of Affymetrix DNA microarray HGU133A/B probesets detecting transcripts with transcription starts at a given distance range, the table shows the number of pairs according to their detection status. Columns are: distance, upper limit of distance range (in bp); total_blast, number of pairs at the distance range for the myoblast sample; aa_blast, fraction of pairs where both probesets did not detect gene expression in the myoblast sample; pp_blast, fraction of pairs where both probesets detected gene expression in the myoblast sample; total_tube, number of pairs at the distance range for the myotube sample; aa_tube, fraction of pairs where both probesets did not detect gene expression in the myotube sample; pp_tube, fraction of pairs where both probes detected gene expression in the myotube sample. This data was used for the graphs in Figure 2B.
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Additional file 3:
Supplementary Table S3. Association between histone H4 hyperacetylation and gene expression in human myoblasts and myotubes. For all pairs of SNP-array probes and gene transcription starts at a given distance range, the table shows the number of pairs of SNP-array probes and DNA microarray probesets according to their detection status. Columns are: distance, upper limit of distance range (in bp); pT_blast, number of pairs where the SNP-array probe detected histone H4 hyperacetylation and the DNA microarray probeset detected gene expression in the myoblast sample; pS_blast, number of pairs where the SNP-array probe detected histone H4 hyperacetylation and the DNA microarray probeset did not detect gene expression in the myoblast sample; aT_blast, number of pairs where the SNP-array probe did not detect histone H4 hyperacetylation and the DNA microarray probeset detected gene expression in the myoblast sample; aS_blast, number of pairs where the SNP-array probe did not detect histone H4 hyperacetylation and the DNA microarray probeset did not detect gene expression in the myoblast sample; pT_tube, number of pairs where the SNP-array probe detected histone H4 hyperacetylation and the DNA microarray probeset detected gene expression in the myotube sample; pS_tube, number of pairs where the SNP-array probe detected histone H4 hyperacetylation and the DNA microarray probeset did not detect gene expression in the myotube sample; aT_tube, number of pairs where the SNP-array probe did not detect histone H4 hyperacetylation and the DNA microarray probeset detected gene expression in the myotube sample; aS_tube, number of pairs where the SNP-array probe did not detect histone H4 hyperacetylation and the DNA microarray probeset did not detect gene expression in the myotube sample. This data was used for the graphs in Figure 3.
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