Table 5

Gene variability by qRT-PCR

Interquartile range
 Full range
 # Outliers
Gene
 UC
 MFS
 MFS/UC
 UC
 MFS
 MFS/UC
 UC
 MFS
COL1A2
 2.9
 1.9
 0.7
 19.2
 66.1
 3.4
 0
 6
ELN
 2.4
 24.3
 10.4
 1190.4
 1687.3
 1.4
 4
 0
FBN1
 1.6
 3.3
 2.0
 4.4
 69.3
 15.6
 0
 2
INHBA
 2.2
 3.7
 1.7
 124.5
 2218.2
 17.8
 4
 3
LMO7
 4.7
 2.0
 0.4
 25.9
 141.1
 5.4
 0
 7
PCOLCE
 2.6
 1.8
 0.7
 5.9
 295.5
 50.3
 0
 5
PDCD10
 1.8
 1.4
 0.8
 103.6
 64.7
 0.6
 1
 6
PLOD2
 2.0
 3.1
 1.5
 16.0
 251.2
 15.7
 1
 3
PTGES
 2.8
 3.7
 1.3
 31.7
 225.0
 7.1
 1
 1
VDR
 3.9
 11.3
 2.9
 230.3
 233.0
 1.0
 1
 0
min
 1.6
 1.4
 0.4
 4.4
 64.7
 0.6
 0.0
 0.0
median
 2.5
 3.2
 1.4
 28.8
 229.0
 6.3
 1.0
 3.0
mean
 2.7
 5.7
 2.2
 175.2
 525.1
 11.8
 1.2
 3.3
max
 4.7
 24.3
 10.4
 1190.4
 2218.2
 50.3
 4.0
 7.0
# > 1

6

8

For the 10 genes validated by qRT-PCR, we summarize variability across 32 UC and 42 MFS samples. Interquartile Range: fold change between the 25th and 75th percentiles. Full Range: fold change between the most extreme pair of samples. #Outliers: number of outliers (triangles in Figure 2). If the data were normally distributed, approximately 1% of the points would so identified, i.e., on average 0.32 per gene for UC and 0.42 per gene for MFS samples.

Yao et al. BMC Genomics 2007 8:319   doi:10.1186/1471-2164-8-319