Table 1

Bacterial strains and plasmids

Strain or plasmid

Relevant property

Reference or origin


Escherichia coli

DH5α

F-, φ80dlacZΔM15 Δ(lacZYA-argF)U169

Invitrogen

deoR recA1 endA1 hsdR17(rk-, mk+)

phoA supE44 λ- thi-1 gyrA96 relA1

S17-1

recA pro hsdR RP4-2-Tc::Mu-Km::Tn7

[45]

Pseudomonas aeruginosa

PAO1

Wild type

[46]

PAO1ΔlasR

PAO1 derivative; ΔlasR::TcR

[47]

PAO1ΔrhlR

PAO1 derivative; ΔrhlR::GmR

[47]

PAO1ΔrhlRΔlasR

PAO1 derivative;

ΔrhlR::GmR

ΔlasR::TcR

[47]

PAO-rpoS

PAO1 derivative; rpoS::GmR

[15]

MSC100

PAO1 derivative; ΔrhlRΔlasR, pBADlasR

This study

MSC110

PAO1 derivative; ΔrhlRΔlasR, pBADrhlR

This study

MSC120

PAO1 derivative; rpoS::GmR, pBADrpoS

This study

Plasmids

pFLP2

Source of Flp recombinase; ApR

[37]

pJN105

araC-pBAD cloned in pBBR1MCS-5; GmR

[41]

pJN105.lasR

lasR in pJN105

[48]

pJN105.rhlR

rhlR in pJN105

This study

pJN105.rpoS

rpoS in pJN105

This study

pSW196

mini-CTX1 based plasmid containing

[40]

araC-pBAD from pBAD30; TcR

pSW196.lasR

lasR in pSW196

This study

pSW196.rhlR

rhlR in pSW196

This study

pSW196.rpoS

rpoS in pSW196

This study


Schuster and Greenberg BMC Genomics 2007 8:287   doi:10.1186/1471-2164-8-287

Open Data