 Research articleA detailed transcript-level probe annotation reveals alternative splicing based microarray platform differencesJoseph C Lee1 1 Genomics Core Laboratory, National Institute of Diabetes & Digestive & Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA 2 Office of the Director, National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA
BMC Genomics 2007, 8:284doi:10.1186/1471-2164-8-284
Additional filesAdditional file 1: Probe alignment failure conditions. Provides examples of probes failing to align for various causes. Format: DOC Size: 27KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 2: Minimum number of probes for reliable Affymetrix summarization measurements. Graph showing the correlation coefficient versus number of probes in a probe set matching to RefSeq in a cross platform comparison between Affymetrix and Agilent. Format: DOC Size: 24KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 3: Alignments and real-time PCR designs for 3 other "discordant" genes: CDC42, CALM1, FMO5. Alignments on the UCSC Genome browser for each platform's probe(set) against relevant AceView transcripts. Real-time PCR primers and probes are included. Format: DOC Size: 365KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 4: Perl and Matlab code used for sequence matching and analysis. The readme file contains descriptions for each script. Format: ZIP Size: 22KB Download file |
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