Additional file 2.
Random hexamer versus gene specific priming using in tact RNA as a template. The 21 genes from the OncotypeDX Breast Cancer assay were assayed using Commercially available, in tact RNA as template. Random hexamer priming (RH) or gene specific pool (GSP) priming in the reverse transcription reaction were compared. Each gene was run in triplicate wells and the data shown represents the average and standard deviation. There is only a modest benefit from using GSP priming versus random priming when the template is of high quality.
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Clark-Langone et al. BMC Genomics 2007 8:279 doi:10.1186/1471-2164-8-279