Comparison of paired whole plate average CT values for all patients. Expression analysis for 761 unique genes required each patient RNA sample to be divided between two 384 well plates. Shown here is the average raw CT value for all wells of data obtained for plate one plotted against the average raw CT obtained for plate two, for each patient. The patient sample which appears to be an outlier was re-assayed through RT-PCR and on repeat analysis, fell into alignment with the other samples. The solid line represents the least squares line fit and the dashed line represents the line of concordance.
Clark-Langone et al. BMC Genomics 2007 8:279 doi:10.1186/1471-2164-8-279