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WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations

Pongsakorn Wangkumhang, Kridsadakorn Chaichoompu, Chumpol Ngamphiw, Uttapong Ruangrit, Juntima Chanprasert, Anunchai Assawamakin and Sissades Tongsima*

BMC Genomics 2007, 8:275  doi:10.1186/1471-2164-8-275

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query regarding this software

Mamoonah Chaudhry   (2011-10-18 15:26)  university of the punjab email

dear author,
one of my friend referred this article to me as i was aiming to do allele specific PCR. i found this software very useful.As i am trying to design primers using this software, maximum Tm difference is quite confusing, as far as my knowledge is concerned, it should be not more than 5 degree c. whenever i try to change it in software, it designs no primers. please give me your email address for direct contact or please help me in this regard.


Competing interests

None declared


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