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WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations

Pongsakorn Wangkumhang, Kridsadakorn Chaichoompu, Chumpol Ngamphiw, Uttapong Ruangrit, Juntima Chanprasert, Anunchai Assawamakin and Sissades Tongsima*

BMC Genomics 2007, 8:275  doi:10.1186/1471-2164-8-275

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query regarding this software

Mamoonah Chaudhry   (2011-10-18 15:26)  university of the punjab email

dear author,
one of my friend referred this article to me as i was aiming to do allele specific PCR. i found this software very useful.As i am trying to design primers using this software, maximum Tm difference is quite confusing, as far as my knowledge is concerned, it should be not more than 5 degree c. whenever i try to change it in software, it designs no primers. please give me your email address for direct contact or please help me in this regard.

thanks

Competing interests

None declared

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