WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations
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* Corresponding author: Sissades Tongsima sissades@biotec.or.th
BMC Genomics 2007, 8:275 doi:10.1186/1471-2164-8-275
query regarding this software
Mamoonah Chaudhry (2011-10-18 15:26) university of the punjab
dear author,
one of my friend referred this article to me as i was aiming to do allele specific PCR. i found this software very useful.As i am trying to design primers using this software, maximum Tm difference is quite confusing, as far as my knowledge is concerned, it should be not more than 5 degree c. whenever i try to change it in software, it designs no primers. please give me your email address for direct contact or please help me in this regard.
thanks
Competing interests
None declared
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