|
Unisequences of Microbotryum violaceum blasting against pheromone receptors. For each of the four unisequences significantly blasting against pheromone receptors: best hits, primer designed for PCR amplification, expected size from the EST sequence, rough amplification size obtained, and mating type of the sporidia that gave amplification products. |
||||||
| Number of ESTs |
Best hits |
Primers |
Contig size obtained from ESTs |
Rough amplification size |
Amplification in sporidia of mating type |
|
|
|
||||||
| Contig588 |
3 |
Rcb1 of Coprinopsis cinerea |
F1: GGAAGGCCATTACAAGAAAGG |
350 |
500 |
A2 only |
| Bbr2 of Schizophyllum commune |
R2: TGTGCTTTTCGCTCTTAGCA |
|||||
| Contig660 |
4 |
Rcb2 of C. cinerea |
F: ACGATTCCAGTAGGCGTGAA |
551 |
800 |
A1 only |
| B alpha 8 of S. commune |
R: CTGCGTCACGATACCTTTCTT |
|||||
| Contig2096 |
3 |
Bbr2 of S. commune |
F: TCCTTTGTCACGACAAGCAC |
213 |
220 |
A1 only |
| Rcb3 of C. cinerea |
R: CCAATTTTCACGCCTACTGG |
|||||
| Singlet pr0aaa87yh06 |
1 |
Rcb1 of C. cinerea |
F: ATCAGAATATGACGGCAGCA |
383 |
600 |
A2 only |
| Bbr2 of S. commune |
R: AAGAAAGGGAACTCCAAATGC |
|||||
|
1 F: Forward primer 2 R: Reverse primer | ||||||
Yockteng et al. BMC Genomics 2007 8:272 doi:10.1186/1471-2164-8-272 |
||||||