BMC Genomics

official impact factor 4.21

Open Access Research article

A high resolution radiation hybrid map of bovine chromosome 14 identifies scaffold rearrangement in the latest bovine assembly

Elisa Marques1, Simon de Givry2, Paul Stothard1, Brenda Murdoch1, Zhiquan Wang1, James Womack3 and Stephen S Moore1*

Author Affiliations

1 Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

2 Unité de Biométrie et d'Intelligence Artificielle INRA, BP 52627, 31326 Castanet-Tolosan, France

3 Department of Veterinary Pathobiology, Texas A & M University, College Station, TX 77843, USA

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BMC Genomics 2007, 8:254 doi:10.1186/1471-2164-8-254

Published: 26 July 2007

Additional files

Additional file 1:

Complete list of markers mapped on BTA14. Summary of markers mapped to 12 K RH BTA14 map, including accession number, RH map position, bovine sequence assembly and human genome coordinates and sequence. Human coordinates that could not be assigned were left blank. Markers highlighted in yellow are those presented in figure 2. Yellow highlighted human coordinates represent small inversions, which if rearranged would be a perfect match with HSA8. BCM-HGSC: Baylor College of Medicine database, IBISS: Interactive Bovine In Silico SNP database, UofA: University of Alberta.

Format: XLS Size: 583KB Download file

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Additional file 2:

Retention frequency pattern graph of markers mapped on BTA14. Graph depicting the range in retention frequency patterns for markers mapped on BTA14 using the 12 K radiation hybrid panel. The dotted blue line represents the average retention frequency value, while the green dotted line represents the line of best fit for the retention frequency values.

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Additional file 3:

RH genotypes for all markers mapped to BTA14 using the 12 K radiation hybrid panel. Screening results for the 180 cell lines in the 12 k panel. "0" represents no amplification of the particular marker in the cell line. "1" indicates amplification of the particular marker in the cell line. "2" represents missing results.

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Additional file 5:

Full image of 12 K RH map of BTA14 compared to the corresponding Btau_3.1 map

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Additional file 4:

List of all markers with inconsistent positions when comparing the 12 k RH BTA14 and Btau_3.1 maps

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Additional file 6:

Full image of 12 K RH map of BTA14 with Homologous Conserved Synteny Blocks from HSA8

Format: JPEG Size: 663KB Download file

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