Table 3 |
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Primers used for probes, southern hybridization and PCR |
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Sequence (5'-3') |
Position |
Comments |
|
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|
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UBA 540F |
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|
UBA TmR |
TCTTCTTCCAAATGACGACCCC |
Exon 5 |
cDNA amplification Sasa-UBA |
|
TAP2.550F |
GCGGGACACCGTCAGGGCAGT |
Exon 5 |
cDNA amplification Sasa-TAP2a/b |
|
TAP2.850R |
CGGCCCCACCAGAGCAGTCAG |
Exon 8 |
cDNA amplification Sasa-TAP2a/b |
|
PSMB8.50F |
GACTTCGTGGGCAGATTCTT |
Exon 2 |
cDNA amplification Sasa-PSMB8 |
|
PSMB8.350R |
TCAGCAGCACTACCAGACAT |
Exon 3 |
cDNA amplification Sasa-PSMB8 |
|
PSMB9b4.590F |
GAGGACTGCCAACAGTTTGTT |
Exon 4 |
cDNA clone (2B4) amplification Sasa-PSMB9 |
|
PSMB9b4.876R |
ATATCATGCTGGCACAATGTT |
3' UTR |
cDNA clone (2B4) amplification Sasa-PSMB9 |
|
UBAlead1.F |
CTGGGAATAGGCCTTCTACAT |
Exon 1 |
Sasa-UBA*0201,*0801,*0901 specific |
|
UBAlead2.F |
AGCCCTACATTCTTCATCTGC |
Exon 1 |
Sasa-UBA*0301,*1001 specific |
|
UBA02.285R |
GTTTGATTGAAGCGGGATTTC |
Exon 2 |
Sasa-UBA*0201 specific |
|
UBA03.281R |
TCTGATTGAAGCGCTGCTTGG |
Exon 2 |
Sasa-UBA*0301 specific |
|
UBA08.280R |
CTGATTGAAGCGCTGCTTGAC |
Exon 2 |
Sasa-UBA*0801 specfic |
|
UBA01.286R |
GTTTGATTGAAACGTGTCTG |
Exon 2 |
Sasa-UBA*0901 specific |
|
UBA10.272R |
TTGAAAGATTGTGAGGTGCT |
Exon 2 |
Sasa-UBA*1001 specific |
|
ssal.phc-004.039-2 |
CTCTCCAGTGACCTGCCACGCTACAGGTTTCTATCCCAGTGGAGTCATGGTGTTCTGGCAGAAAGATGGA |
MHC Non-classical |
|
|
ssal.rgb-532.282-r |
GCCAATGTGCTCATGGCTATAGTCATCATTGTGTCTGTAGTCTTGATACTCACTGTCCTATTCAAGTATT |
MHC Non-classical |
|
|
ssal.rgb-505.101 |
GCTACCATCGGCTCAAAGAGGAACTCTTTGAGGACTGATTAGGAATCACACAGCTGTCAGAGAGAGAGAC |
TAP2 |
|
|
ssal.rgb-516.329 |
TCAACCTGTACCACATGCAGGAGGACGGCTGGATAAAGGTGTGTAAGGAGGACGTTTCAGAGCTGATC |
PSMB8 |
|
|
ssal.rgb-523.298 |
GTTGATGGACAGGAAGGGGAGCTACTACAAACTAAGAGAGAGACTGTTCACGGAAGACGACACGTCACAT |
TAP2 |
|
|
ssal.rgb-550.202 |
TTCATTGGCTATGAGTCGAGATGGTTCCAGCGGAGGCGTGGCCTACCTTGTCACTATTGATGAAAAGGGT |
PSMB9 |
|
|
868O01_F1 |
GGCTTGCGGAGTAGAACACTTGAAAAAGAA |
GAP-primers |
|
|
868O01_F2 |
GCAACCAATACACTGCAGTATTTCTACACGGCCACTTCTGGTATAGATAAC |
GAP-primers |
|
|
868O01_F3 |
TCAATCAAACTGGAGGTGAGTAGAGACAGA |
GAP-primers |
|
|
714P22_R1 |
CAGCGGAAACGCAACAAACACAAGAATAACTTACTAACAAATTAGAATCA |
GAP-primers |
|
|
714P22_R2 |
AGCATACAACCATGCCGACAACCATC |
GAP-primers |
|
|
92I04_R1 |
AACATTTCAAGGTGGTGAAACTATTCACAT |
GAP-primers |
|
|
92I04_R2 |
AATTAGTTGTTGTTCACTATGTTAATTAGT |
GAP-primers |
|
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|
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Lukacs et al. BMC Genomics 2007 8:251 doi:10.1186/1471-2164-8-251 |
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