Table 2

Defense-related genes induced after P. rapae feeding in cultivars Rivera and Christmas Drumhead.

Rivera

Christmas Drumhead

Probe Identification and Putative Function

AGI Code

24 h

48 h

72 h

24 h

48 h

72 h

Genes only induced in Rivera

Basic endochitinase

At3g12500

1.12

2.54*

2.62

1.06

1.37

1.94

Cup-shaped cotyledon1 protein (CUC1)

At3g15170

1.07

1.63

2.21*

1.11

1.26

1.92

DNA-binding protein

At1g49950

1.35

0.34

2.25*

1.66

1.81

1.50

Glutathione S-transferase

At1g27130

1.02

2.64*

2.01*

1.17

1.21

1.98

Glycosyl hydrolase 1 (BG1)

At1g52400

1.14

2.79

11.00*

2.71

-¹

6.45

Lectin

At5g35950

0.89

2.51*

1.32

1.02

1.28

1.79

MYB transcription factor

At1g71030

1.16

2.07*

1.60

1.26

1.06

1.79

Telomere repeat-binding protein

At3g46590

0.95

2.48*

1.41

1.24

0.87

1.51

Terpene synthase

At4g16730

1.13

4.15*

2.82*

1.16

1.26

1.97

Trypsin inhibitor

At2g43520

1.19

1.74

3.70*

1.51

-¹

2.44

Genes only induced in Christmas Drumhead

Cytochrome P450 71B15 (CYP71B15)

At3g26830

1.01

1.90

-¹

1.32

1.33

3.51*

ERF domain protein 9 (ERF9)

At5g44210

1.08

1.47

-¹

1.19

1.23

2.01*

Glutathione S-transferase (ERD9)

At1g10370

1.04

1.55

1.33

1.54

1.77

2.10*

IAA-amino acid hydrolase 3 (IAR3)

At1g51760

1.13

1.76

1.42

1.05

1.23

2.01*

Lectin

At3g16400

1.13

1.86

1.51

1.58

2.03*

1.78

Legume lectin

At1g53070

0.98

1.73

2.03

1.31

1.67

4.21*

MADS-box protein (AGL74)

At1g48150

1.42

0.44

1.40

1.87

2.79*

1.54

Terpene synthase

At5g23960

-¹

-¹

-¹

1.27

1.15

4.29*

Tryptophan synthase β subunit 2 (TSB2)

At4g27070

1.03

1.50

1.19

1.30

2.00

2.94*

Vegetative storage protein 2 (VSP2)

At5g24770

-¹

1.10

6.96

2.68

3.49

16.20*

Genes induced in both cultivars

Allene oxide synthase (AOS)

At5g42650

1.71

3.27*

2.46*

1.72

2.08*

3.50*

Coronatine-responsive tyrosine aminotransferase

At4g23600

2.28

28.34*

10.11*

7.70*

7.89*

14.70*

Cysteine proteinase (RD21A)

At1g47128

1.02

2.06*

2.83*

1.83

2.66*

3.96*

Cytochrome b5

At2g46650

1.07

3.02*

1.71

1.18

3.13*

3.68

Cytochrome P450 79B2 (CYP79B2)

At4g39950

1.47

3.23*

4.45*

1.37

1.74

7.18*

Cytochrome P450 83B1 (CYP83B1)

At4g31500

1.59

19.92*

9.38*

3.23*

10.40*

10.99*

Ethylene-responsive element-binding protein

At5g07580

1.99

6.88*

1.89

3.17*

5.73*

7.37*

Glutathione S-transferase 6 (GST6)

At2g47730

1.06

2.03*

1.50

1.44

2.82*

2.40*

Hydroperoxide lyase (HPL1)

At4g15440

1.26

2.88*

2.05*

1.51

2.86*

3.75*

Lectin

At3g16470

1.71

3.67

15.33*

3.36

5.93*

7.21*

Lectin kinase

At3g45410

2.57

14.61*

4.53*

8.38*

3.88*

7.04*

Lipoxygenase (LOX2)

At3g45140

4.74

29.91*

29.27*

11.65*

11.89*

14.53*

MYB transcription factor (MYB49)

At5g54230

1.17

4.36*

1.96

1.13

1.63

6.29*

Myrosinase-associated protein

At1g54020

1.46

4.28*

5.01*

3.06*

2.22*

6.54*

Plant defensin-fusion protein (PDF2.3)

At2g02130

1.11

1.34

2.16*

1.76

2.92*

2.19*

Polygalacturonase inhibiting protein 2 (PGIP2)

At5g06870

1.09

3.37*

5.99*

3.04

5.31*

20.16*

Terpene synthase

At1g61120

1.69

3.48*

5.32*

3.37*

2.44*

3.04*

Trypsin inhibitor

At2g43530

1.59

2.66*

4.34*

3.25

2.11*

4.62*

Trypsin/protease inhibitor

At1g72290

3.03

38.70*

23.75*

13.18*

24.37*

34.11*

Tryptophan synthase α subunit (TSA1)

At3g54640

1.19

15.18*

6.72*

2.73

17.34*

12.69*

Tryprophan synthase β subunit 1 (TSB1)

At5g54810

0.94

5.48*

3.43*

1.34

3.91*

4.47

Relative changes in gene expression after challenge with P. rapae larvae were measured in Rivera and Christmas Drumhead plants. Mean expression ratios are calculated from three biologically independent replicates. Only genes known to be involved in defense in A. thaliana are shown. *Fold change ≥ 2 with a P-value < 0.05. ¹70-mer oligonucleotide did not hybridize in any of the three replicates. AGI, Arabidopsis Genome Initiative.

Broekgaarden et al. BMC Genomics 2007 8:239   doi:10.1186/1471-2164-8-239