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Comparative genomic hybridizations reveal absence of large Streptomyces coelicolor genomic islands in Streptomyces lividans

Karthik P Jayapal1 email, Wei Lian1,3 email, Frank Glod2,4 email, David H Sherman2 email and Wei-Shou Hu1 email

Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Ave. SE., Minneapolis, MN 55455, USA

Life Sciences Institute, Departments of Medicinal Chemistry, Chemistry, Microbiology & Immunology, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI 48109, USA

Abbott Bioresearch Center, 100 Research Drive, Worcester, MA 01605, USA

Fonds National de la Recherche, 6 rue Antoine de Saint-Exupéry, L-1017 Kirchberg, Luxembourg

author email corresponding author email

BMC Genomics 2007, 8:229doi:10.1186/1471-2164-8-229

Published: 10 July 2007

Additional files

Additional file 1:

PCR verification for genomic islands – primers used and gel images. Details of primers chosen for PCRs to verify the absence of genomic islands in S. lividans and gel electrophoresis images of PCR products in each case.

Format: PDF Size: 147KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional file 2:

Table of microarray data and GI designations of genes probed. Contains comparative genomic microarray hybridization ratios from three replicate experiments, designations of presence/absence in S. lividans, GI designations, CAI values and maximum/average expression in transcriptome data for each gene.

Format: CSV Size: 484KB Download file


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