Table 3 |
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|
List of candidate genes studied and marker information |
||||||
|
Gene type |
Gene ID |
Marker type |
Forward primer |
Reverse primer |
PCR product (bp) |
LG |
|
|
||||||
|
MdMYB10 |
Indel |
CGAGTGCTAATAGCAACC |
CCTCTGTTGGCCGAATACAC |
786 |
9 |
|
|
AATGGCCTACCACATCATTG |
1,182 |
|||||
|
MdMYB9 |
SSR |
TCCATAACCTTCTTGGTAACAG |
AAGGAGAATCGGCTTTCGAT |
329 |
NP |
|
|
MdMYB17 |
SSR |
TGCTCCTCTCTAGCTATTGCATAAT |
AAGACTCACAAACTAGCTGTCAAAT |
205 |
14 |
|
|
Transcription factors |
MdBHLH33 |
SNP |
TGGCATTCAGCTAAAACCT AAT |
TCTCTGGCACCCTCTCTTGG |
145 |
NP |
|
MdANL2 |
- |
- |
- |
- |
- |
|
|
MdBHLH3 |
SSR |
CAACTCCCCTTATTCTTCTTCTCTC |
CACCTGACCTTCTCTCTACCTCTAC |
170 |
11 |
|
|
MdMYB12 |
SSR |
CTCGGCAATCGGTAAAGCTA |
TATGAACAGTGAAACCCTAACCCTA |
152 |
3 |
|
|
MdTTG1 |
SNP |
CACCCGAACCCCAACGG CCA |
CTCTCCAGCTCCGCCACTG |
527 |
15 |
|
|
|
||||||
|
MdBAN |
SNP |
GTTGGGTGAGCTAGAGATATTC |
GATACTGCTGGGAACGTCA GGA |
472 |
10 |
|
|
MdCHS |
SNP |
GAGGAAGTTCGCAAGGCTCA |
CCCCATTCCTTGATGGCTA TGA |
343 |
NP |
|
|
Flavonoid pathway |
MdDFR |
SNP |
CTGTGAATGTGGAGGAG GAC |
TCAGTTTTCTCTGCCGGAAT |
616 |
UL |
|
MdF3H |
SNP |
GGCGTGAAATTGTGACCTACTA TTT |
TGACCACAAAAGCTCCTTCC |
392 |
NP |
|
|
MdANS |
SNP |
TCAGACCTAAAGATGAC CTT |
TCCTGACCTTGTCCATGAGC |
1,449 |
NP |
|
|
|
||||||
|
NP: not polymorphic in the M.9 × 'Robusta 5' population. UL: unlinked. LG: linkage group. The SNP position in the allele-specific primer is underlined. Extra mismatch in the allele-specific primer is shown in italics. No marker could be designed for MdANL2, because neither SNPs nor SSRs were detected. |
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|
Chagné et al. BMC Genomics 2007 8:212 doi:10.1186/1471-2164-8-212 |
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