Methodology article
A DArT platform for quantitative bulked segregant analysis
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* Corresponding author: Andrzej Kilian andrzej@diversityarrays.com
1 Diversity Arrays Technology P/L, 1 Wilf Crane Cr., Yarralumla, Canberra ACT 2600, Australia
2 Triticarte P/L, 1 Wilf Crane Cr., Yarralumla, Canberra ACT 2600, Australia
3 NSW Agricultural Genomics Centre and NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, PMB, Wagga Wagga NSW 2650, Australia
4 Tasmanian Institute of Agricultural Research, University of Tasmania, PO Box 46, Kings Meadows TAS 7249, Australia
BMC Genomics 2007, 8:196 doi:10.1186/1471-2164-8-196
Published: 28 June 2007Additional files
Additional File 1:
Influence of the hybridization contrast between parental alleles on the precision of estimating allele-frequency equality. PDF file with a chart displaying the relationship between the apparent allele-frequency differences, measured by comparing two identical aliquots of a 1:1 mixture of Steptoe and Morex, and the hybridization contrasts between alternative alleles. All markers present in a Steptoe/Morex DArT map [20] were included in this figure. The SD of groups of markers in allelic-contrast bins of 0.5 units on the log2 [cy3/cy5] scale are included.
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Additional File 2:
Comparison between two bulking strategies. PDF file with a chart displaying, for each of 490 markers, the difference of the Dayton allele frequency between Al-tolerant and Al-sensitive bulks of 20 Dayton/Zhepi2 DH plants (Δ %), measured using two alternative methods: (1) by pooling representations prepared from individual DNA samples (horizontal axis), and (2) by preparing two representations from genomic-DNA pools. The dotted line denotes equality between the two alternative methods.
Format: PDF Size: 69KB Download file
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