Definition of the zebrafish genome using flow cytometry and cytogenetic mapping

doi:10.1186/1471-2164-8-195

BMC Genomics

Volume 8

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Freeman JL
Adeniyi A
Banerjee R
Dallaire S
Maguire SF
Chi J
Ng BL
Zepeda C
Scott CE
Humphray S
Rogers J
Zhou Y
Zon LI
Carter NP
Yang F
Lee C

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Freeman JL
Adeniyi A
Banerjee R
Dallaire S
Maguire SF
Chi J
Ng BL
Zepeda C
Scott CE
Humphray S
Rogers J
Zhou Y
Zon LI
Carter NP
Yang F
Lee C
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Research article

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping

Jennifer L Freeman¹^,2 , Adeola Adeniyi¹ , Ruby Banerjee³ , Stephanie Dallaire¹ , Sean F Maguire³ , Jianxiang Chi³ , Bee Ling Ng³ , Cinthya Zepeda⁴ , Carol E Scott³ , Sean Humphray³ , Jane Rogers³ , Yi Zhou⁵ , Leonard I Zon⁵ , Nigel P Carter³ , Fengtang Yang³^* and Charles Lee¹^,2^*

¹Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA

²Harvard Medical School, Boston, Massachusetts 02115, USA

³Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

⁴Centro de Ciencias Genomicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico

⁵Howard Hughes Medical Institute and Division of Hematology/Oncology, Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA

author email corresponding author email* Contributed equally

BMC Genomics 2007, 8:195doi:10.1186/1471-2164-8-195


Published:	27 June 2007

Additional files

Additional file 1:

A table listing 510 BAC clones assigned to a unique LG chromosome location by cytogenetic mapping and the predicted LG chromosome location indicated by four zebrafish genome databases (as of August 2006). The 510 BAC clones were assigned to a chromosome, chromosome arm, and region within each chromosome arm as observed with cytogenetic mapping. The current genome assembly position (as of May 2007) for each BAC clone was then integrated with the cytogenetic mapping data to further order the BAC clones within each chromosomal region. It should be noted that LG chromosomes 3, 5, 7, 8, 17, 18, 21, 22, and 25 appear to be inverted.

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Additional file 2:

A table summarizing the concordance of the chromosomal locations assigned by cytogenetic mapping with the most current build of the zebrafish genome assembly (Zv6-as of May 2007).

Format: PDF Size: 12KB Download file

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Additional file 3:

A table listing the BAC clones observed to hybridize to multiple locations within the zebrafish genome using FISH.

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Additional file 4:

A table summarizing the sequencing status of the 510 BAC clones cytogenetically mapped to unique chromosomal locations in this study.

Format: PDF Size: 13KB Download file

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