BMC Genomics

official impact factor 4.21

Open Access Methodology article

New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures

Marta Campo Dell'Orto1, Andrea Zangrando1, Luca Trentin1, Rui Li2, Wei-min Liu2, Geertruy te Kronnie1, Giuseppe Basso1 and Alexander Kohlmann2*

Author Affiliations

1 University of Padua, Laboratory of Molecular Diagnostic, Department of Pediatric Oncology, Via Giustiniani 3, 35128, Padua, Italy

2 Roche Molecular Systems, Inc., Department of Genomics and Oncology, Pleasanton, CA, USA

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BMC Genomics 2007, 8:188 doi:10.1186/1471-2164-8-188

Published: 22 June 2007

Additional files

Additional File 1:

Supplementary Data. This file contains supplementary figures with additional comments explaining details of analysis, results, and interpretation.

Format: DOC Size: 1.3MB Download file

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Additional File 2:

This Excel file contains further details about each total RNA isolation method, including cRNA quality and quantity values as well as microarray quality and quantity values for each experiment.

Format: XLS Size: 33KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional File 3:

This Excel file contains details about each total RNA isolation method and leukemia classification details for each CEL file. All microarray raw data (*.cel files) are available online through the Gene Expression Omnibus database with the series accession number GSE7757.

Format: XLS Size: 25KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data