Table 1

Peptidases of Entamoeba histolytica

Protein name

Clan, family, subfamily

Accession No.

Protein length

Active site residues

Remarks

Name, NCBI


Cysteine peptidases


1

EhCP-A1

CA, C1, A

XP_650156

XM_645064

315

Q112C118H259N279

SP: 13, Pro: 80, TM: -

cysteine protease 1

2

EhCP-A2

CA, C1, A

XP_650642

XM_645550

315

Q112C118H259N279

SP: 13, Pro; 80, TM: -

cysteine proteinase 2

3

EhCP-A3

CA, C1, A

XP_653254

XM_648162

308

Q111C115H251N271

SP: 13, Pro: 79, TM: -

cysteine proteinase acp1

4

EhCP-A4

CA, C1, A

XP_656602

XM_651510

311

Q112C118H253N273

SP: 20, Pro: 73, TM: -

cysteine proteinase

5

EhCP-A5

CA, C1, A

XP_650937

XM_645845

318

Q113C119H261N271

SP: 20, Pro: 72, TM: -

cysteine proteinase

6

EhCP-A6

CA, C1, A

XP_657364

XM_652272

320

Q115C122H261N281

SP: 17, Pro: 79, TM: -

cysteine proteinase

7

EhCP-A7

CA, C1, A

XP_648996

XM_643904

315

Q112C118H259N279

SP: 13, Pro: 80, TM: -

cysteine protease 8

8

EhCP-A8

CA, C1, A

XP_657446

XM_652354

317

Q116C122H260N280

SP: 15, Pro: 82, TM: -

cysteine protease 9

9

EhCP-A9

CA, C1, A

XP_655675

XM_650583

297

Q126C132H269N290

SP: 17, Pro: 90, TM: -

cysteine protease 10

10

EhCP-A10

CA, C1, A

XP_651147

XM_646598

420

Q185C191H336N357

SP: 18, Pro: 148, TM: -

cysteine protease 17

11

EhCP-A11

CA, C1, A

XP_651690

XM_646598

324

Q118C124H-N287

SP: 17, Pro: 79, TM: -

cysteine protease 19

12

EhCP-A12

CA, C1, A

XP_653823

XM_648731

317

not identified, N281

SP: 14, Pro: 83, TM: -

cysteine proteinase

13

EhCP-A13

CA, C1, A

not annotated

250 (IS)

Q125C130

SP: 18, Pro: 108

14

EhCP-B1

CA, C1, A

XP_651581

XM_646489

426

Q105C101H308N328

SP: 15, Pro: 106, hydroph. C-term.

cysteine proteinase 7

15

EhCP-B2

CA, C1, A

AAO03568

431

Q145C151H308S328

SP: 15, Pro: 106, GPI

cysteine protease 11

16

EhCP-B3

CA, C1, A

XP_656747

XM_651655

474

Q155C161H304N324

SP: 16, Pro: 107, TM: 444–466

cysteine protease 12-related

17

EhCP-B4

CA, C1, A

XP_648501

XM_643409

379

Q153C159H302N322

SP: 16, Pro: 105, TM: 355–377 or GPI

cysteine protease 13

18

EhCP-B5

CA, C1, A

XP_652671

XM_647579

434

Q151C157H311N326

SP: 12, Pro: 108, GPI

cysteine protease 14-related

19

EhCP-B6

CA, C1, A

XP_652465

XM_647373

300

Q84C90H232N252

SP: 14, Pro: 55, hydroph. C-term.

cysteine protease 15

20

EhCP-B7

CA, C1, A

XP_650400

XM_645308

650

Q-C171H312N332

SP: 18, Pro: 144, hydroph. C-term.

cysteine protease 16

21

EhCP-B8

CA, C1, A

XP_651049

XM_645957

473

Q110C156H329N249

SP: 15, Pro: 105, GPI

cysteine protease 18

22

EhCP-B9

CA, C1, A

XP_652993

XM_647901

446

Q161C167H328N348

SP: 19, Pro: 112, hydroph. C-term

cysteine protease

23

EhCP-B10

CA, C1, A

XP_648306

XM_643214

372 (IS)

Q67C76H244N264

hydroph. C-term.

cysteine protease

24

EhCP-B11

CA, C1, A

XP_648013

XM_642921

133 (IS)

cysteine protease 11-related

25

EhCP-C1

CA, C1, A

XP_654453

XM_649361

586

Q70C76H-N345

SA: 12–34

hypothetical protein

26

EhCP-C2

CA, C1, A

XP_656632

XM_651540

567

Q87C93H306N326

SA: 27–49

hypothetical protein

27

EhCP-C3

CA, C1, A

XP_655128

XM_650036

572

Q94C100H322N337

SA: 17–39

hypothetical protein

28

EhCP-C4

CA, C1, A

XP_655800 XM_650708

502

Q32C38H246N271

SP: 15

hypothetical protein

29

EhCP-C5

CA, C1, A

XP_654800 XM_649708

557

Q90C96H302N327

SA: 20–42

hypothetical protein

30

EhCP-C6

CA, C1, A

XP_651553 XM_646461

557

Q93C99H293N-

SA: 14–36

hypothetical protein

31

EhCP-C7

CA, C1, A

XP_657273 XM_652181

595

Q89C95H297N322

SA: 19–41

hypothetical protein

32

EhCP-C8

CA, C1, A

XP_655479 XM_652181

627

Q91C96H317N366

SA: 29–51

hypothetical protein

33

EhCP-C9

CA, C1, A

XP_655011 XM_649919

518

not identified

SA: 12–34

hypothetical protein

34

EhCP-C10

CA, C1, A

XP_654829 XM_649737

530

Q87C93H299N324

SA: 15–37

hypothetical protein

35

EhCP-C11

CA, C1, A

XP_648083 XM_642991

526

not identified

SA: 20–42

hypothetical protein

36

EhCP-C12

CA, C1, A

XP_650829 XM_645737

473

not identified

SA: 26–48, TM: 449–471

hypothetical protein

37

EhCP-C13

CA, C1, A

XP_656556 XM_651464

564

Q89C95H266N290

SA: 21–43

hypothetical protein

38

EhCALP1

CA, C2

XP_649922 XM_644830

591

not identified

SP: -, TM: -

calpain-like cysteine protease

39

EhCALP2

CA, C2

XP_657312 XM_652220

473

Q51C57H206N227

SP: -, TM: -

calpain family cysteine protease

40

EhUBHY

CA, C19

XP_657356 XM_652264

444

not identified

SP: -, TM: -

peptidase

41

EhUBP

CA, C19

XP_654028 XM_648936

352

N30C35H330D348

SP: -, TM: -

ubiquitin-specific protease

42

EhUCH

CA, C19

XP_655880 XM_650788

386

N37C42H345D360

SP: -, TM: -

ubiquitin carboxyl-terminal hydrolase

43

EhUlp1-1

CA, C48

XP_650529 XM_645437

197

H95D112Q146C152

SP: -, TM: -

Ulp1 protease

44

EhUlp1-2

CA, C48

XP_651052 XM_645960

538

H399D435Q482C488

SP: -, TM: -

Ulp1 protease

45

EhUlp1-3

CA, C48

XP_657158 XM_652066

285

H174D191Q234C240

SP: -, TM: -

Ulp1 protease

46

EhAUTO1

CA, C54

XP_651386 XM_646294

325

Y71C100D251H253

SP: -, TM: -

peptidase

47

EhAUTO2

CA, C54

XP_653798 XM_648706

364

Y93C103D278H280

SP: -, TM: -

peptidase

48

EhAUTO3

CA, C54

XP_652043 XM_646951

364

Y92C103D279H281

SP: -, TM: -

hypothetical protein

49

EhAUTO4

CA, C54

XP_656724 XM_651632

348

Y92C112D265H267

SP: -, TM: -

hypothetical protein

50

EhOTU

CA, C65

XP_654013 XM_648921

259

D105C108H212

SP: -, TM: -

OTU-like cysteine protease


Aspartic peptidase


51

EhAsP22-1

AD, A22, A

XP_654079 XM_648987

340

D178D223

SP:24 or TM: 7–26 + 7 × TM

Intramembrane protease

52

EhAsP22-2

AD, A22, A

XP_652820 XM_647728

316

D157, D209

SP: 19 or TM: 2–19 + 8 × TM

Signal peptide peptidase

53

EhAsP22-3

AD, A22, A

XP_657563 XM_652471

320

D157D205

SA:7–29 + 8 xTM

Signal peptide peptidase

54

EhAsP22-4

AD, A22, A

XP_653696 XM_648604

396

D245D318

SP: -, 7 × TM

Presenilin 1-related peptidase


Serine peptidase


55

EhSP9-1

SC, S9, C

XP_655265 XM_650173

653

S514D595H627

SP: 15, TM: -

dipeptidyl-peptidase

56

EhSP9-2

SC, S9, C

XP_655222 XM_650130

665

S526D607H639

SP: 15, TM: -

dipeptidyl-peptidase

57

EhSP9-3

SC, S9, C

XP_656380 XM_651288

656

S516D599H632

SP: -, TM: -

prolyl oligopeptidase

58

EhSP9-4

SC, S9, C

XP_649111 XM_644019 XP_648413 XM_643321 XP_655473 XM_650381

669

not identified

SP: 16, TM: -

dipeptidyl-peptidase

59

EhSP9-5

SC, S9

XP_655676 XM_650584

102 (IS)

SP: -, TM: -

prolyl oligopeptidase family

60

EhSP26-1

SF, S26, B

XP_653142 XM_648050

189

S65H106

SP: -, TM: 34–56, 163–185

signal peptidase (signalase)

61

EhSP26-2

SF, S26

XP_651791 XM_646699

121

not identified

SP: -, TM: -

microsomal signal peptidase

62

EhSP28-1

SC, S28

XP_656762 XM_651670

457

S165D395H421

SP: 15, TM: -

serine peptidase

63

EhSP28-2

SC, S28

XP_648991 XM_643899

480

S165D418H444

SP: 15, TM: -

serine peptidase

64

EhSP28-3

SC, S28

XP_652089 XM_646997

466

S155D404H431

SP: -, TM: -

serine peptidase


Metallo peptidases


65

EhMP1-1

MA, M1

XP_652558 XM_647466

827

H295E296H299E318Y481

SP: -, TM: -

aminopeptidase

66

EhMP3-1

MA, M3

XP_649877 XM_644785

675

H463E464H467E493

SP: -, TM: -

oligopeptidase A

67

EhMP3-2

MA, M3

XP_649600 XM_644508

710

H498E499H502E527

SP: -, TM: -

oligopeptidase A

68

EhMP8-1

MA, M8

XP_655394 XM_650302

643

H206E207H210H270M281

SP: 18, TM: 605–627

leishmaniolysin-related peptidase

69

EhMP8-2

MA, M8

XP_652632 XM_647540

662

H207E208H211H267M278

SP: -, TM: 598–620

leishmaniolysin-related peptidase

70

EhMP48-1

MA, M48, A

XP_648770 XM_643678

416

H274E275H278E353

SA: 4–21 + 6 × TM

CAAX prenyl protease

71

EhMP16-1

ME, M16, C

XP_654849 XM_649757

970

H59E62H63E137E158

SP: -, TM: -

Zn-dependent peptidase, eupitrilysin

72

EhMP24-1

MG, M24, A

XP_651539 XM_646447

409

H165D186D197H266E299E394

SP: -, TM: -

methionine aminopeptidase

73

EhMP24-2

MG, M24, B

XP_657085 XM_651993

471

H244D265D276H354H358H365E401E441

SP: -, TM: -

Xaa-Pro dipeptidase

74

EhMP24-3

MG, M24, B

XP_654211 XM_649119

563

H364D384D396H461H465H470E491E505

SP: -, TM: -

aminopeptidase

75

EhMP24-4

MG, M24, B

XP_650646 XM_645554

559

H346D366D378H443H447H452E473E487

SP: -, TM: -

aminopeptidase

76

EhMP24-5

MG, M24, B

XP_649980 XM_644888

589

H369D389D401H466H475E496E510

SP: -, TM: -

aminopeptidase

77

EhMP24-6

MG, M24, B

XP_653331 XM_648239 XM_649891 XM_644799

371

not identified

SP: -, TM: -

peptidase

78

EhMP18-1

MH, M18

XP_656618 XM_651526

435

H79D81D222E258E259D307H402

SP: -, TM: -

aminopeptidase

79

EhMP18-2

MH, M18

XP_650466 XM_645374

431

H81D82D228E262E263D311H401

SP: -, TM: -

aspartyl aminopeptidase

80

EhMP20-1

MH, M20, B

XP_656428 XM_651336

379

H75D133E163E164D186H353

SP: -, TM: -

peptidase T

81

EhMP20-2

MH, M20, B

XP_650152 XM_645060

401

H79D139E172E173D195H376

SP: -, TM: -

peptidase T

82

EhMP20-3

MH, M20, C

XP_656545 XM_651453 XP_655596 XM_650504 XP_652163 XM_647071

516

H95D97D134E165E166D192H490

SP: -, TM: -

aminoacyl-histidine dipeptidase

83

EhMP20-4

MH, M20, C

XP_655616 XM_650524

505

H95D97D-E154E155D181H477

SP: -, TM: -

aminoacyl-histidine dipeptidase

84

EhMP22-1

MK, M22

XP_652292 XM_647200

335

unknown

SP: -, TM: -

glycoprotein endopeptidase

85

EhMP49-1

M, M49

XP_654273 XM_649181

645

H412E413H417E467

SP: -, TM: -

dipeptidyl-peptidase III

86

EhU48-1

U, U48

XP_656466 XM_651374

216

H274E275H278E353

SA: 4–26 + 6 × TM

CAAX prenyl protease


Tillack et al. BMC Genomics 2007 8:170   doi:10.1186/1471-2164-8-170

Open Data