p53-dependent induction of target genes. Panels A-H show site-specific qPCR ChIP results on left with standard deviation, where significance at p ≤ 0.05 is designated by an asterisk (*). Center tile depicts gene map of predicted p53 binding site location and the sequence relative to annotated genes, along with exon structure and direction of transcription as per NCBI MapViewer conventions. Right tile represents RT-PCR gel image of each mapped target gene. RT-PCR analyses were performed multiple times for each target gene and gel images are representative of consistent expression levels. mRNA levels of each gene in wild-type naïve PC12 cells were compared to 7-day NGF (50 ng/mL) differentiated wild-type and three anti-p53 shRNA expressing PC12 cells (p53#3low, p53#2mid, p53#4mid). ImageJ quantitation, normalized to naïve RNA levels, is depicted under each band, while PCR cycle number is depicted on right of gel image. GAPDH expression levels are shown in A and represent a typical loading control along with no-RT negative control.
Brynczka et al. BMC Genomics 2007 8:139 doi:10.1186/1471-2164-8-139