Table 2

Oligonucleotides used for the introduction of amino acid substitutions in PtdMTP1

Primer name

Position

Sequence

Substitution


C30Sf

72-102

TGGAGGAAGCAAGGGAaGCGGGGAAGCACCT

C30S

C30Sr

102-72

AGGTGCTTCCCCGCtTCCCTTGCTTCCTCCA

C30S

C35Sf

87-118

TGCGGGGAAGCACCTTgTGGATTTTCAGAT

C35S

C35Sr

118-87

ATCTGAAAATCCAcAAGGTGCTTCCCCGCA

C35S

C64Sf

175-205

ATATCCGTGGCACTTTcTATAGTCTTCATG

C64S

C64Sr

205-175

CATGAAGACTATAgAAAGTGCCACGGATAT

C64S

G118Af

343-372

CAATCTTATGcATTTTTTAGGATTGAGATT

G118A

G118Ar

372-343

AATCTCAATCCTAAAAAATgCATAAGATTG

G118A

H260Df

764-793

AAGGAGCTTATCTCgATGTACTTGGGGATT

H260D

H260Dr

793-764

AATCCCCAAGTACATcGAGATAAGCTCCTT

H260D

D264Ef

775-804

CTCCATGTACTTGGGGAaTCCATCCAGAGT

D264E

D264Er

804-775

ACTCTGGATGGAtTCCCCAAGTACATGGAG

D264E

D264Af

775-804

CTCCATGTACTTGGGGcTTCCATCCAGAGT

D264A

D264Ar

804-775

ACTCTGGATGGAAgCCCCAAGTACATGGAG

D264A

D288Ef

847-876

GAGTGGAAGATAATTGAaCTGATCTGCACC

D288Ef

D288Er

876-847

GGTGCAGATCAGtTCAATTATCTTCCACTC

D288Ef

D288Af

847-876

GAGTGGAAGATAATTGcTCTGATCTGCACC

D288A

D288Ar

876-847

GGTGCAGATCAGAgCAATTATCTTCCACTC

D288A

C291Sf

855-885

ATAATTGATCTGATCTcCACCCTAATCTTT

C291S

C291Sr

885-855

AAAGATTAGGGTGgAGATCAGATCAATTAT

C291S

C357Sf

1053-1083

AAGATTCTCTTGGCTTcTCATGTGAAAATC

C357S

C357Sr

1083-1053

GATTTTCACATGAgAAGCCAAGAGAATCTT

C357S


For each oligonucleotide the position on the PtdMTP1 coding sequence is shown, together with the sequence and the target mutation. Nucleotides written in lower case were mutated with respect to the original sequence.

Montanini et al. BMC Genomics 2007 8:107   doi:10.1186/1471-2164-8-107

Open Data