Dose-response linearity check by LBM. Dose-response linearity of the Affymetrix GeneChip by the LBM (liver-brain mix) sample set. Five samples, i.e. mixtures of mouse liver and brain at ratios of 100:0, 75:25, 50:50, 25:75 and 0:100, were spiked with GSC and measured by Affymetrix GeneChips Mouse430-2. Signals were normalized by the Percellome method as described in the text. Line graphs are in (a) copy numbers and (b) ratio to 50:50 sample for the top 1,000 probe sets with coefficient of correlation (R2) closest to 1 among those having 1 copy or more per cell in the 50:50 sample (19,979 probe sets out of 45,101). The number of probe sets with R2 > 0.950 was 8,655, and R2 > 0.900 was 11,719.
Kanno et al. BMC Genomics 2006 7:64 doi:10.1186/1471-2164-7-64