Table 1

Summary of pyrosequence data from the Soudan Mine


Red Sample
Black Sample

Number of Sequences
334,386
388,627
Total Length of Sequences
35,439,683 bp
38,502,057 bp
Average Length of Sequences
106.0 bp
99.1 bp
Average Quality Score1
26.2
25.8
Skew2
2.53
2.44

1 The quality score of each base was provided by 454 Life Sciences, and is analogous to the Phred score of Sanger Sequencing methods [26]. The value cited here is the average of the mean quality score per sequence.

2The skew was calculated by comparing the dinucleotide frequencies within each library. A similar analysis performed on Bacterial and Eukaryotic genomes sampled at random yielded entropies of 2.63 and 2.66 respectively.

Edwards et al. BMC Genomics 2006 7:57   doi:10.1186/1471-2164-7-57