Table 4

Results of validation by qRT-PCR alongside detection of differential expression by microarray demonstrating qualitative agreement.

qPCR Validation

Gene Name

2ΔΔCT

2ΔΔCT standard deviation

qPCR p-value

qPCR

Direction of Imprinted Expression

Porcine Spotted Long Oligo

Human Short Oligo

Porcine Short Oligo


PEG10

785544.42

9.3

<0.0001

Yes

Paternal

Yes

Yes

Yes

PLAGL1

758.32

1.4

<0.0001

Yes

Paternal

No

Yes

Yes

SGCE

450.90

2.1

<0.0001

Yes

Paternal

No

Yes

Yes

IGF2

24.53

1.9

0.0010

Yes

Paternal

No

Yes

Yes

DLX5

7.53

1.5

0.0065

Yes

Maternal

No

No

No

DCN

5.04

1.6

0.0168

Yes

Maternal

No

No

No

GRB10

1.38

2.2

0.1913

No

Maternal

No

No

No

CD81

0.98

1.6

0.2026

No

Maternal

No

No

No

COMMD1

0.86

2.8

0.3265

No

Maternal

No

No

No

CDKN1C

0.79

6.7

0.4283

No

Maternal

No

No

No

IGF2R

0.74

1.3

0.2119

No

Maternal

No

No

No


Tsai et al. BMC Genomics 2006 7:328   doi:10.1186/1471-2164-7-328

Open Data