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Open AccessResearch article

Deep and comparative analysis of the mycelium and appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray methods

Malali Gowda1* email, RC Venu1* email, Mohan B Raghupathy1 email, Kan Nobuta2 email, Huameng Li1 email, Rod Wing3 email, Eric Stahlberg4 email, Sean Couglan5 email, Christian D Haudenschild6 email, Ralph Dean7 email, Baek-Hie Nahm8 email, Blake C Meyers2 email and Guo-Liang Wang1 email

Department of Plant Pathology, Ohio State University, Columbus, OH 43210, USA

Department of Plant and Soil Sciences, University of Delaware, DE 19711, USA

Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA

Ohio Supercomputer Center, The Ohio State University, Columbus, OH 43210, USA

Agilent Technologies Inc, Little Falls Site, 2850 Centerville Road, Delaware 19711, USA

Solexa, Inc. 25861 Industrial Blvd, Hayward, CA, 94545, USA

Fungal Genomics Laboratory, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27606, USA

Department of Biological Science, Myongji University, Kyonggido, 449728, Korea

author email corresponding author email* Contributed equally

BMC Genomics 2006, 7:310doi:10.1186/1471-2164-7-310

Published: 8 December 2006

Additional files

Additional file 1:

Appressorial and mycelial MPSS tags, microarray up/down regulated genes and KOG analysis results

Format: XLS Size: 6.3MB Download file

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Additional file 2:

Novel appressorial and mycelial MPSS and RL-SAGE tags.

Format: XLS Size: 2.1MB Download file

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Additional file 3:

Alternative sense and antisense transcript tags for selected genes.

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Additional file 4:

Antisense transcript tags for annotated genes.

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Additional file 5:

Annotated genes with sense and antisense tags specifically and commonly present in mycelia and appressoria.

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Additional file 6:

List of alternative transcript tags from MPSS and EST.

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Additional file 7:

Alternative tags for selected genes in appressoria and mycelia identified by MPSS.

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Additional file 8:

Common alternative tags detected by RL-SAGE and MPSS.

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Additional file 9:

List of top 20 highly and specifically up regulated and down regulated genes in appressoria and in mycelia of M. grisea detected by oligoarray and MPSS.

Format: XLS Size: 29KB Download file

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Additional file 10:

The up-regulated genes (4,649) in appressoria identified by oligoarray analysis were subjected for KOGs analysis based on the putative function in the KOGs protein database. Functional classification and percentage of genes represented in appressorial tissue is shown.

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Additional file 11:

The up-regulated genes (3,784) in mycelia identified by oligoarray analysis were subjected for KOGs analysis based on the putative function in the KOGs protein database. Functional classification and percentage of genes represented in mycelia tissue is shown.

Format: PPT Size: 41KB Download file

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