Additional file 4.

Microarray data for gene groups previously implicated in host response to malaria. Intensity values (median-subtracted, normalized, log2 scale) are shown for mouse genes (rows) represented on the microarray, in several functional categories thought to be associated with malaria infection. Samples (columns) are chronologically ordered by organ. Left, heat maps showing relative intensity (%-tile Int.), and significance levels at day 0 (D0) or responding to infection (R.I.), which shows whether the corresponding gene was expressed significantly different between resistant and susceptible mice prior to infection, over infection or both. Categories examined included cell adhesion, toll-like receptors, immune response (cytokines, chemokines and their receptors), interferon responsive genes, acute phase proteins, erythroid-associated transcripts, complement activation, glycolysis and hemostasis. The final digits of its GO identifiers in parenthesis indicate GO-BP categories. Genes in each group were ordered using cosine-angle correlation and hierarchical clustering on the log-ratios. The hypergeometric distribution was used to determine whether there was significant overlap between genes in these categories and those chosen by the linear model. This analysis supports the role of toll-like receptors; cytokines, chemokines and their receptors; interferon responsive genes; acute phase proteins and complement activation in the PbA CM model.

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Lovegrove et al. BMC Genomics 2006 7:295   doi:10.1186/1471-2164-7-295