Research article
Huntingtin gene evolution in Chordata and its peculiar features in the ascidian Ciona genus
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* Corresponding authors: Graziano Pesole graziano.pesole@biologia.uniba.it - Elena Cattaneo elena.cattaneo@unimi.it
1 Dipartimento di Scienze Biomolecolari e Biotecnologie, Università di Milano, Milano, Italy
2 Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Bari, Italy
3 Department of Pharmacological Sciences and Center of Excellence on Neurodegenerative Diseases, University of Milano, Milano, Italy
BMC Genomics 2006, 7:288 doi:10.1186/1471-2164-7-288
Published: 8 November 2006Additional files
Additional file 1:
Huntingtin amino acid alignment. Amino acid alignment, in fasta format, of the htt proteins of chordates.
Format: TXT Size: 38KB Download file
Additional file 2:
Exon size of chordate huntingtin genes. Length (in bp) is reported only for proten-coding exons.
Format: XLS Size: 22KB Download file
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Additional file 3:
Huntingtin EST clones of Ciona. Ciona EST clones corresponding to partial huntingtin transcripts, and similarity to the htt mRNA sequence determined in this study
Format: DOC Size: 44KB Download file
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Additional file 4:
Schematic map of the C. intestinalis huntingtin transcript amplification strategy. Amplified fragments are reported as blue boxes; amplified and cloned fragments are reported as shaded boxes. EST clones, listed in 3, are reported as white boxes. Arrows indicate the position of inner RACE primers. The 5'- and 3'-UTR regions are shown as a thick line.
Format: JPEG Size: 262KB Download file
Additional file 5:
Protein sequence Accession Numbers (AC), genome assemblies and genomic coordinates of the region used in huntingtin annotation. Genomic coordinates refer to a sequence with about 10000 additional nucleotides upstream and downstream the region of initial similarity to the protein sequence
Format: DOC Size: 38KB Download file
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Additional file 6:
Huntingtin nucleotide alignment. Nucleotide alignment, in fasta format, of the htt protein-coding sequences of chordates. The alignment was obtained by"back-translation" of the amino acid alignment.
Format: TXT Size: 113KB Download file