BMC Genomics

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454 sequencing put to the test using the complex genome of barley

Thomas Wicker, Edith Schlagenhauf, Andreas Graner, Timothy J Close, Beat Keller and Nils Stein*

BMC Genomics 2006, 7:275 doi:10.1186/1471-2164-7-275

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Research article   Open Access

Identification of expressed resistance gene-like sequences by data mining in 454-derived transcriptomic sequences of common bean (Phaseolus vulgaris L.)

Zhanji Liu, Mollee Crampton, Antonette Todd, Venu Kalavacharla BMC Plant Biology 2012, 12:42 (23 March 2012)

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Sequencing of BAC pools by different next generation sequencing platforms and strategies

Stefan Taudien, Burkhard Steuernagel, Ruvini Ariyadasa, Daniela Schulte, Thomas Schmutzer, Marco Groth, Marius Felder, Andreas Petzold, Uwe Scholz, Klaus FX Mayer, Nils Stein, Matthias Platzer BMC Research Notes 2011, 4:411 (14 October 2011)

Research   Open Access

Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features

Leonor Palmeira, Bénédicte Machiels, Céline Lété, Alain Vanderplasschen, Laurent Gillet Virology Journal 2011, 8:406 (16 August 2011)

Research article   Open Access

New genomic resources for switchgrass: a BAC library and comparative analysis of homoeologous genomic regions harboring bioenergy traits

Christopher A Saski, Zhigang Li, Frank A Feltus, Hong Luo BMC Genomics 2011, 12:369 (18 July 2011)

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Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8

Max Farnbacher, Thomas Jahns, Dirk Willrodt, Rolf Daniel, Rainer Haas, Alexander Goesmann, Stefan Kurtz, Gabriele Rieder BMC Genomics 2010, 11:335 (27 May 2010)

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Transcriptome sequencing in an ecologically important tree species: assembly, annotation, and marker discovery

Thomas L Parchman, Katherine S Geist, Johan A Grahnen, Craig W Benkman, C Alex Buerkle BMC Genomics 2010, 11:180 (16 March 2010)

Research   Open Access

Genomic and small RNA sequencing of Miscanthus × giganteus shows the utility of sorghum as a reference genome sequence for Andropogoneae grasses

Kankshita Swaminathan, Magdy S Alabady, Kranthi Varala, Emanuele De Paoli, Isaac Ho, Dan S Rokhsar, Aru K Arumuganathan, Ray Ming, Pamela J Green, Blake C Meyers, Stephen P Moose, Matthew E Hudson Genome Biology 2010, 11:R12 (3 February 2010)

Genomic data together with sequencing of tissue specific small RNA libraries reveals insights into the genome content, small RNA repertoire and evolutionary origins of the grass Miscanthus × giganteus.

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Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek)

Sithichoke Tangphatsornruang, Prakit Somta, Pichahpuk Uthaipaisanwong, Juntima Chanprasert, Duangjai Sangsrakru, Worapa Seehalak, Warunee Sommanas, Somvong Tragoonrung, Peerasak Srinives BMC Plant Biology 2009, 9:137 (24 November 2009)

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Global characterization of Artemisia annua glandular trichome transcriptome using 454 pyrosequencing

Wei Wang, Yejun Wang, Qing Zhang, Yan Qi, Dianjing Guo BMC Genomics 2009, 10:465 (9 October 2009)

Methodology article   Open Access Highly Accessed

Deep sampling of the Palomero maize transcriptome by a high throughput strategy of pyrosequencing

Julio C Vega-Arreguín, Enrique Ibarra-Laclette, Beatriz Jiménez-Moraila, Octavio Martínez, Jean Vielle-Calzada, Luis Herrera-Estrella, Alfredo Herrera-Estrella BMC Genomics 2009, 10:299 (6 July 2009)

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Next-generation pyrosequencing of gonad transcriptomes in the polyploid lake sturgeon (Acipenser fulvescens): the relative merits of normalization and rarefaction in gene discovery

Matthew C Hale, Cory R McCormick, James R Jackson, J Andrew DeWoody BMC Genomics 2009, 10:203 (29 April 2009)

Research article   Open Access

The lipoxygenase gene family: a genomic fossil of shared polyploidy between Glycine max and Medicago truncatula

Jin Hee Shin, Kyujung Van, Dong Hyun Kim, Kyung Do Kim, Young Eun Jang, Beom-Soon Choi, Moon Young Kim, Suk-Ha Lee BMC Plant Biology 2008, 8:133 (23 December 2008)

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High quality draft sequences for prokaryotic genomes using a mix of new sequencing technologies

Jean-Marc Aury, Corinne Cruaud, Valérie Barbe, Odile Rogier, Sophie Mangenot, Gaelle Samson, Julie Poulain, Véronique Anthouard, Claude Scarpelli, François Artiguenave, Patrick Wincker BMC Genomics 2008, 9:603 (16 December 2008)

By combining 454 GSFLX and Solexa-Illumina technologies to sequence medically important microbes, quality is maintained while lowering the cost and time taken to sequence whole genomes.

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Low-pass shotgun sequencing of the barley genome facilitates rapid identification of genes, conserved non-coding sequences and novel repeats

Thomas Wicker, Apurva Narechania, Francois Sabot, Joshua Stein, Giang TH Vu, Andreas Graner, Doreen Ware, Nils Stein BMC Genomics 2008, 9:518 (31 October 2008)

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Assessing the feasibility of GS FLX Pyrosequencing for sequencing the Atlantic salmon genome

Nicole L Quinn, Natasha Levenkova, William Chow, Pascal Bouffard, Keith A Boroevich, James R Knight, Thomas P Jarvie, Krzysztof P Lubieniecki, Brian A Desany, Ben F Koop, Timothy T Harkins, William S Davidson BMC Genomics 2008, 9:404 (28 August 2008)

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Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda)

Aaron R Jex, Min Hu, D Timothy J Littlewood, Andrea Waeschenbach, Robin B Gasser BMC Genomics 2008, 9:11 (11 January 2008)

Research article   Open Access

Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation

Ian Armstead, Lin Huang, Julie King, Helen Ougham, Howard Thomas, Ian King BMC Genomics 2007, 8:283 (20 August 2007)

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Accuracy and quality of massively parallel DNA pyrosequencing

Susan M Huse, Julie A Huber, Hilary G Morrison, Mitchell L Sogin, David Welch Genome Biology 2007, 8:R143 (20 July 2007)

Error rates were estimated for the Roche GS20 massively parallel pyrosequencing system, and several factors were identified that can be used to remove low-quality reads, improving the accuracy to 99.75% or better.