|
Real-time RT-PCR analysis of significant genes (p < 0.05) identified in microarray data normalized with and/or without EC dilution series. |
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| Name |
Full Name |
GenBank |
Overall Treatment Effect |
Pairwise Comparisons |
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|
|
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| With EC |
Without EC |
# of Flagged Spots |
With EC |
Without EC |
RT-PCR |
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| p-value |
p-value |
Sample |
Ref. |
Comparison |
foldchange |
p-value |
foldchange |
p-value |
foldchange |
p-value |
|||
|
|
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| Differentially expressed using both the EC and non-EC normalization |
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|
|
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| Adcy6 |
adenylate cyclase 6 |
NM_007405 |
0.0004 |
0.0068 |
6 |
0 |
100 mg/kg |
-1.43 |
0.0003 |
-1.34 |
0.0037 |
||
| Chk |
choline kinase |
NM_013490 |
0.0039 |
0.0223 |
1 |
0 |
100 mg/kg |
-1.45 |
0.0041 |
-1.39 |
0.0214 |
-2.39 |
0.0122 |
| Cyp2b10 |
Cytochrome P450, 2b10, phenobarbitol inducible, type b |
NM_009998 |
0.0000 |
0.0000 |
8 |
20 |
100 mg/kg |
5.10 |
0.0000 |
3.93 |
0.0000 |
3.76 |
0.0001 |
| Cyp2b10 |
NM_009998 |
10 mg/kg |
2.95 |
0.0000 |
2.92 |
0.0000 |
1.04 |
0.8600 |
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| Cyp2b9 |
cytochrome P450, 2b9, phenobarbitol inducible, type a |
NM_010000 |
0.0000 |
0.0000 |
15 |
22 |
100 mg/kg |
8.29 |
0.0000 |
8.55 |
0.0000 |
3.04 |
0.0965 |
| Cyp2b9 |
NM_010000 |
10 mg/kg |
3.22 |
0.0000 |
3.56 |
0.0000 |
2.74 |
0.0052 |
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| Ephx1 |
epoxide hydrolase 1, microsomal; Eph1, Eph-1, AI195553 |
NM_010145 |
0.0181 |
0.0021 |
0 |
0 |
100 mg/kg |
1.33 |
0.0486 |
1.39 |
0.0004 |
1.46 |
0.0350 |
| Gadd45a |
growth arrest and DNA damage inducible |
NM_007836 |
0.0000 |
0.0000 |
17 |
0 |
100 mg/kg |
1.66 |
0.0003 |
1.62 |
0.0024 |
||
| Gadd45b |
growth arrest and DNA-damage-inducible 45 beta |
NM_008655 |
0.0000 |
0.0000 |
25 |
1 |
100 mg/kg |
1.92 |
0.0000 |
1.93 |
0.0000 |
||
| Gdf15 |
growth differentiation factor 15 |
NM_011819 |
0.0000 |
0.0002 |
10 |
1 |
100 mg/kg |
2.83 |
0.0000 |
2.57 |
0.0000 |
||
| gstm2 |
glutathione S-transferase, mu 2 |
NM_008183 |
0.0054 |
0.0002 |
0 |
0 |
100 mg/kg |
1.39 |
0.0313 |
1.40 |
0.0000 |
1.50 |
0.0013 |
| Gstm3 |
glutathione S-transferase, mu 3 |
J03953 |
0.0000 |
0.0160 |
6 |
24 |
100 mg/kg |
2.16 |
0.0000 |
2.21 |
0.0131 |
||
| Lbp |
Lipopolysaccharide binding protein |
NM_008489 |
0.0379 |
0.0051 |
1 |
25 |
100 mg/kg |
1.39 |
0.0104 |
1.38 |
0.0037 |
1.60 |
0.0345 |
| Lpin1 |
lipin 1 |
NM_015763 |
0.0039 |
0.0037 |
0 |
3 |
100 mg/kg |
-1.94 |
0.0009 |
-1.66 |
0.0037 |
||
|
|
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| Differentially expressed using EC normalization only |
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|
|
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| AhR |
rat Ah receptor |
NM_013464 |
0.0039 |
0.0436 |
0 |
0 |
100 mg/kg |
1.48 |
0.0423 |
1.42 |
0.0676 |
2.03 |
0.0003 |
| Mpg |
N-methylpurine-DNA glycosylase |
NM_010822 |
0.041698 |
0.3782 |
25 |
25 |
10 mg/kg |
1.30 |
0.0420 |
1.13 |
0.3911 |
1.42 |
0.0096 |
| Olfr1161 |
olfactory receptor 1161 |
NM_146848 |
0.03003 |
0.0800 |
25 |
25 |
10 mg/kg |
1.30 |
0.0420 |
1.12 |
0.3921 |
1.02 |
0.9618 |
| sult1c2 |
sulfotransferase family 1C, member 2 |
NM_026935 |
0.0350 |
0.0572 |
18 |
25 |
100 mg/kg |
1.55 |
0.0044 |
1.56 |
0.0214 |
1.60 |
0.0161 |
| Tieg1 |
TGFB inducible early growth response 1 |
NM_013692 |
0.0062 |
0.1318 |
0 |
0 |
100 mg/kg |
-1.64 |
0.0011 |
-1.45 |
0.0627 |
-2.88 |
0.0008 |
| Ube2b |
ubiquitin conjugating enzyme |
NM_009458 |
0.0350 |
0.0897 |
0 |
0 |
100 mg/kg |
-1.23 |
0.0358 |
-1.16 |
0.7402 |
-1.56 |
0.0058 |
|
|
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| Differentially expressed using non-EC normalization only |
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|
|
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| cd8b |
CD8 antigen, beta chain |
NM_009858 |
0.2327 |
0.0436 |
25 |
25 |
100 mg/kg |
-1.10 |
0.8557 |
-1.16 |
0.0158 |
1.40 |
0.3272 |
| Dusp6 |
dual specificity phosphatase 6 |
NM_026268 |
0.0181 |
0.0107 |
16 |
0 |
100 mg/kg |
1.39 |
0.0852 |
1.41 |
0.0486 |
1.40 |
0.0752 |
| Gsta2 |
Glutathione S-transferase, alpha 2 (Yc2) |
NM_008182 |
0.0681 |
0.0160 |
0 |
0 |
100 mg/kg |
1.84 |
0.2849 |
2.26 |
0.0353 |
4.02 |
0.0026 |
| Gsta4 |
Glutathione S-transferase, alpha 4 |
NM_010357 |
0.0045 |
0.0006 |
4 |
4 |
100 mg/kg |
1.32 |
0.1312 |
1.41 |
0.0037 |
1.20 |
0.2200 |
| Idi1 |
isopentenyl-diphosphate delta isomerase |
NM_145360 |
0.0835 |
0.0436 |
0 |
0 |
100 mg/kg |
-1.58 |
0.1234 |
-1.57 |
0.0254 |
-1.16 |
0.3523 |
| Pitpn |
phosphatidylinositol transfer protein |
NM_008850 |
0.0969 |
0.0004 |
20 |
15 |
100 mg/kg |
1.24 |
0.0624 |
1.23 |
0.0000 |
1.07 |
0.3100 |
| Por |
NADPH cytochrome p450 oxidoreductase |
NM_008898 |
0.0402 |
0.0341 |
0 |
0 |
100 mg/kg |
1.66 |
0.0806 |
1.55 |
0.0489 |
1.45 |
0.0064 |
| Rbp1 |
retinol binding protein 1, cellular |
NM_011254 |
0.0101 |
0.0094 |
0 |
7 |
100 mg/kg |
1.34 |
0.2364 |
1.49 |
0.0029 |
1.13 |
0.3600 |
| Ugt1a6 |
UDP glycosyltransferase 1 family, polypeptide A6 |
XM_135531 |
0.0942 |
0.0063 |
0 |
0 |
100 mg/kg |
1.35 |
0.1044 |
1.38 |
0.0019 |
-1.06 |
0.4810 |
|
|
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| Not differentially expressed |
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|
|
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| Anpep |
aminopeptidase N |
NM_008486 |
0.0070 |
0.0223 |
5 |
9 |
100 mg/kg |
1.20 |
0.4022 |
1.20 |
0.3851 |
||
| Anpep |
NM_008486 |
10 mg/kg |
-1.20 |
0.1456 |
-1.14 |
0.9253 |
-1.04 |
0.6932 |
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| Cyp2a4 |
Cytochrome P450, 2a4 (phenobarbitol inducer) |
NM_009997 |
0.0818 |
0.0045 |
24 |
24 |
100 mg/kg |
1.19 |
0.2364 |
1.14 |
0.3851 |
||
| Cyp2a4 |
NM_009997 |
10 mg/kg |
1.12 |
0.2511 |
-1.07 |
0.4999 |
1.32 |
0.0789 |
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| mpg |
N-methylpurine-DNA glycosylase |
NM_010822 |
0.0417 |
0.3782 |
25 |
25 |
100 mg/kg |
1.09 |
0.8894 |
1.03 |
0.9890 |
-1.15 |
0.1757 |
| Olfr1161 |
olfactory receptor 1161 |
NM_146848 |
0.0300 |
0.0800 |
25 |
25 |
100 mg/kg |
1.13 |
0.8471 |
1.06 |
0.9867 |
-2.87 |
0.0386 |
Yauk et al. BMC Genomics 2006 7:266 doi:10.1186/1471-2164-7-266 |
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