Figure 8.

An example of a more typical EC positional hybridization pattern. The array data were derived from a hybridization reaction of one RNA sample included in the EC-normalization validation exercise summarized in Tables 1–3. Cy5 (experiment) and Cy3 (reference) values are plotted for two EC spot concentrations (6.25 and 50 μM) across the 48 subgrids on the array and ordered as shown in Figure 7. A comparison of the average of the Cy5/Cy3 ratios of Grids #10–28 with the combined average of Grids # 1–9 and 11–48 showed no significant difference (t-test, two-tailed, p = 0.390 and p = 0.393 for 50 and 6.25 μM, respectively)