|
Primer sequences and PCR conditions |
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| Gene |
Primer sequences |
Ta (C°) |
Cycles |
|
|
|||
| GAPDH |
F: 5'-caccaccatggagaaggctgg-3' R: 5'-ccaaagttgtcatggatgacc-3' |
65 |
30 |
| COL3A1 |
F: 5'-agcctccaactgctccta-3' R: 5'-gtccgggtctacctgatt-3' |
56 |
30 |
| ENO2 |
F: 5'-gtctgctgctcaaggtcaac-3' R: 5'-tccaggcaagcagaggaatc-3' |
54 |
30 |
| FGF18 |
F: 5'-cttcctgctgctgtgcttcc-3' R: 5'-cactccttgctggtgccatc-3' |
57 |
30 |
| JUN |
F: 5'-gctatctaggtggagttg-3' R: 5'-gcacatgccacttgatac-3' |
46 |
30 |
| LHX2 |
F: 5'-tgaaggacagcctggtctac-3' R: 5'-gagctgcttcaagtccttgg-3' |
56 |
30 |
| MEIS1 |
F: 5'-gctgttccagcatctaacac-3' R: 5'-tgttgctgaccgtccattac-3' |
50 |
30 |
| MEIS2 |
F: 5'-gatcacgccgttatgttgcc-3' R: 5'-gctggagttcgagtgatgag-3' |
49 |
30 |
| PBX3 |
F: 5'-caggaagcaggacatcgg-3' R: 5'-ttggctctgtaatctgagtgtt-3' |
56 |
30 |
| PDGFRL |
F: 5'-gtggagctaccctgcgtatc-3' R: 5'-ctgggagaaggtacaaagagttc-3' |
60 |
35 |
| PRICKLE1 |
F: 5'-aggtacggtattgccagtctt-3' R: 5'-cgaacactgcaacttcacctc-3' |
60 |
35 |
| SAT |
F: 5'-tcactcgccgaggttccttg-3' R: 5'-acagcagcactcctcactcc-3' |
53 |
30 |
| SHB |
F: 5'-gtttaatggcaacgagaagcg-3' R: 5'-tcctcacagccacgggatag-3' |
60 |
35 |
| TIMP2 |
F: 5'-ggaaacgacatttatggcaacc-3' R: 5'-acccagtccatccagaggc-3' |
60 |
30 |
| TLX1 |
F: 5'-acctcactggcctcaccttc-3' R: 5'-cagaccacggctgcagattc-3' |
57 |
30 |
| TOP1 |
F: 5'-gaaggaacagctagcagatg-3' R: 5'-agaactctgcctcttgagac-3' |
50 |
30 |
| UACA |
F: 5'-cactgaatgacacgttagcca-3' R: 5'-atcctgcacctttctcatgct-3' |
60 |
35 |
|
Ta, annealing temperature. | |||
Crijns et al. BMC Genomics 2006 7:232 doi:10.1186/1471-2164-7-232 |
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