Table 4

In situ localization of TRPC3, TRPC5 and TRPC6 transcripts in different mouse organs

tissue

TRPC3

TRPC5

TRPC6


Skeletal muscle

• sarcolemma

+

neg.

±

• sarcoplasmic reticulum

neg.

neg.

neg.

• myofibrils

neg.

neg.

neg.


Heart

• cardiac muscle cells

+

neg.

±

• blood vessel endothelium

neg.

neg.

neg.


Aorta

• Tunica muscularis

+

+

n. d.

• endothelium

+

neg.

n. d.


small intestine

• smooth muscle layer

neg.

±

neg.

• submucosa

+

±

±

• enterocytes of the villi

++

+

++

• enterocytes of the crypts

+++

+++

++


Kidney

• glomeruli

±

neg.

neg.

• distal tubule

+++

±

++

• proximal tubule

neg.

neg.

neg.


Lung

• bronchial epithel

+++

+

++

• pneumocytes

+

±

+

• blood vessel endothelium

+

neg.

+


Liver

• hepatocytes

+++

++

++

• epithel of the Ductus interlobularis bilifer

+++

++

++


Spleen

• red pulp

++

neg.

neg.

• white pulp

neg.

++

++


Pancreas

• exocrine acini

+++

+++

+++

• islet of Langerhan's

neg.

neg.

neg.

• duct system

++

neg.

neg.

• blood vessel endothelium

neg.

neg.

neg.


testis

• leydig cells

+

+

++

• sertoli cells

neg.

neg.

neg.

• spermatogonia/spermatocytes

neg.

neg.

+

• spermatozoa

neg.

neg.

++


Epididymis

• epithelium of the Ductuli efferentes testis

+++

++

++

• epithelium of the Ductus epididymidis

+

++

++

• spermatozoa

neg.

neg.

neg.


Uterus

• endometrium – glandular epithelial cells

++

+++

+++

• myometrium

neg.

++

+


fallopian tube

• smooth muscle layer

neg.

±

neg.

• tubal epithelium

+

+

+


Ovary

• cortex

- granulosa cells

++

+

++

- theca cells

neg.

±

+

- corpus luteum

neg.

±

+

• medulla

neg.

neg.

+


CNS

• spinal ganglia

+++

+

n. d.

• cortex

++

±

+

• hippocampus

+

neg.

+

• ventricle ependym

+++

±

++

• basal ganglia

++

neg.

++

• cerebellum

- Purkinje cells

++

±

+++

- Stratum granulosum

+

neg.

+


The in situ hybridization technique was applied to tissue sections as shown in Fig. 3. The obtained signals were classified into six categories: negative (neg.), very weak (±), weak (+), moderate (++), strong (+++) or not determined (n. d.).

Kunert-Keil et al. BMC Genomics 2006 7:159   doi:10.1186/1471-2164-7-159

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