Figure 4.

Contig containing the trp1 gene. PCR screening identified trp1 sequences in BAC clone b03TI073AG01, near one end of this contig. A RFLP was developed by shotgun sequencing of clone b04TI008AG07, near the other end of the contig. Genetic mapping shows these markers are about 3 cM apart, confirming the utility of this contig spanning approximately 2 Mb.