Hyperspectral microarray scanning: impact on the accuracy and reliability of gene expression data

doi:10.1186/1471-2164-6-72

BMC Genomics

Volume 6

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Timlin JA
Haaland DM
Sinclair MB
Aragon AD
Martinez MJ
Werner-Washburne M

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Aragon AD
Martinez MJ
Werner-Washburne M
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Research article

Hyperspectral microarray scanning: impact on the accuracy and reliability of gene expression data

Jerilyn A Timlin¹ , David M Haaland¹ , Michael B Sinclair¹ , Anthony D Aragon² , M Juanita Martinez² and Margaret Werner-Washburne²

¹Sandia National Laboratories*, P.O. Box 5800, Albuquerque, NM, 87185, USA

²Lovelace Respiratory Research Institute, 2435 Ridgecrest SE, Albuquerque, NM, 87108, USA

author email corresponding author email

BMC Genomics 2005, 6:72doi:10.1186/1471-2164-6-72


Published:	11 May 2005

Additional files

Additional File 1:

Spectral shifts Cy5 upon incorporation into cDNA MCR extracted spectral profiles of Cy5-dCTP (blue trace) and Cy5-cDNA (green trace). Spectra profiles were obtained from MCR analysis of hyperspectral images from two individual spotted arrays manufactured in-house containing spots of only Cy5 bound to dCTP and Cy5 incorporated into cDNA. Spectral traces are normalized for maximum intensity equal to one

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