Table 2

Appearance of PCR product following gel electrophoresis

Total Clones

Singlet

Doublet

Smear

No Product Visible


Amplification of 2558 Clones

2558

2098 (82%)

117 (5%)

6 (<1%)

337 (13%)

Re-amplification of 183 Clones previously amplified with "No Product Visible"

183

121 (66%)

14 (8%)

0

48 (26%)


Cumulative

2558

2219 (87%)

131 (5%)

6 (<1%)

202 (8%)


Note: PCR-amplified fragments were visualized on ethidium bromide stained 1% agarose, 1 × TBE gels. Approximately 13% of PCR products could not be visualized following the first amplification due to product yield below the threshold of ethidium bromide detection; 54% of these (183) were re-amplified.

Dana et al. BMC Genomics 2005 6:5   doi:10.1186/1471-2164-6-5

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