Figure 1.

Polyacrylamide gels from electromobility shift assays. Polyacrylamide gels showing the decrease in amounts of protein complex with labelled oligomer as the concentration of the competing non-labelled oligomer increases (lanes marked x5 through x25). Lane marked ā€™Cā€™ has no competitor and represents the basal levels of labelled complex. The measure of displacement of the labelled oligomer is expressed as a ratio of radio-labelled product, for each lane, divided by the basal value, presented in Table 2. Comparison of allele specific DNA-protein complex stability is a ratio of the highest competitor concentration (x25) of each of the two alleles. Thus for rs1799722 (A) allele A binds proteins 1.66 times better than allele G and for rs2528521 (B) allele A binds proteins 2.62 times better than allele G (Table 2, extreme right column).

Mottagui-Tabar et al. BMC Genomics 2005 6:18   doi:10.1186/1471-2164-6-18
Download authors' original image