Table 2

ASAP PCR conditions. The thermalcycler parameters used to generate ASAP amplicons in successive rounds of PCR are presented.
ASAP PCR conditions
1. Touchdown PCR
 Temperature (Centigrade)
 Duration (Minutes)
Denaturation
 94
 4
10 Cycles

Denaturation
 94
 0:40
Annealing
 55 – 0.5/cycle
 0:40
Extension
 72
 0:40
25 Cycles

Denaturation
 94
 0:40
Annealing
 50
 0:40
Extension
 72
 0:40
Final extension
 72
 7
2. Touchdown PCR
Denaturation
 94
 4
10 Cycles

Denaturation
 94
 0:40
Annealing
 52 – 0.5/cycle
 0:40
Extension
 72
 0:40
25 Cycles

Denaturation
 94
 0:40
Annealing
 47
 0:40
Extension
 72
 0:40
Final extension
 72
 7
3. Touchdown – extension PCR
Denaturation
 94
 4
10 Cycles

Denaturation
 94
 0:40
Annealing
 52 – 0.5/cycle
 0:40
Extension
 72
 1:00 + .05/cycle
25 Cycles

Denaturation
 94
 0:40
Annealing
 47
 0:40
Extension
 72
 1:30 + 0.05/cycle
Final extension
 72
 10

Dhingra and Folta BMC Genomics 2005 6:176   doi:10.1186/1471-2164-6-176