Figure 4.

Active site region of ACE-like proteins. The active site regions of AnoACEs, the N and C domains of human ACE, D. melanogaster ANCE, ACER and ANCE-3, and Apis 3929 were aligned using CLUSTALX [36]. Amino acids shown to be required for activity in mammalian ACE are highlighted. The histidines in the HExxH motif are required for zinc coordination, whereas the glutamate is required for catalysis. The downstream glutamate is required for zinc coordination. This is replaced by glutamine in ANCE-3.