Table 3 |
||||
|
PCR primer sequences used to amplify DNA (D) or cDNA (C) templates for mutation screening. The order of the amplicons listed is the same as in Table 1. |
||||
|
Gene symbol |
Amplicon size (bp) |
Template |
Forward primer (5' to 3') |
Reverse primer (5' to 3') |
|
|
||||
|
Mc1r |
579 |
D |
gaggatccttcctgacaagactatgtcca |
aacggctgtgtgcttgtagtagg |
|
Mc1r |
446 |
D |
tcgtctccagcaccctctttatc |
gagtcgacgatcaccaggagcacagcagca |
|
Mc1r |
527 |
D |
gcacacttctaatggagagtg |
ggctcaggtagagacatgcc |
|
Zfp111 |
352 |
D |
ccagagagaaataatgcccc |
caagaaccctgggctctcc |
|
Zfp111 |
327 |
D |
ggttcagtcaggtctcacac |
caggattgatataatgctcc |
|
Scnm1 |
394 |
D |
aggcacagcgtctcaaagtattgt |
atagggtagtaggggtcaccactc |
|
Scnm1 |
484 |
D |
ggcaagaagcatttgtccagtaag |
tttctcagaatacaatctggagagc |
|
Scnm1 |
372 |
D |
tgagttcccgtcagccaggacttc |
ctcactcaccttcggagggtaagg |
|
Usp29 |
300 |
D |
ccttactcatcgacaagttatc |
tggaggaggatggttctgtctt |
|
Usp29 |
336 |
D |
gggtctgctggcaccaaaaggt |
ggcaacaagtcagtggtaact |
|
Usp29 |
298 |
D |
gggtcttcaagaggttccagag |
gagcctgtaattctgaagatca |
|
Zim1 |
298 |
D |
ggaagaagacaggggataattcc |
ggagcgctctgtggtgttgtag |
|
Zim1 |
298 |
D |
atcttcgggtcaaacatcagca |
gtagtgtgtgaggaagtatgaga |
|
Zim1 |
302 |
D |
tggagagtgtaacaagtgcttc |
ggtgcttgagaagggctactttg |
|
Zim1 |
302 |
D |
cccggagtgtgggaaagtcttc |
ggtgaatcagcagggtagccagt |
|
Myd88 |
429 |
D |
ggctggcaggagacttaagg |
caggaagcacgtttcctcac |
|
Myd88 |
196 |
D |
cacccttctcttctccacag |
gcccacctattctacctagg |
|
Myd88 |
497 |
D |
ccttctgcagaggctgattg |
ccaaagcaggcctaagcttac |
|
Myd88 |
245 |
D |
cgtggtcctaataccacacc |
ggaggcaagcggaagaacac |
|
Ap2s1 |
424 |
D |
gccttgtctgtacctgtctc |
ggacgagcaggcaggttggtc |
|
Capsl |
372 |
D |
gaggtaaacctagggcttctg |
ctgccacgctgtcaataccg |
|
Capsl |
344 |
D |
gcacatgcatttctccatgg |
gctttgtaaggctctgaacc |
|
Antxr1 |
419 |
D |
gagaatgggagatgaagttgg |
gttcacctagcactttgtgg |
|
Antxr1 |
335 |
D |
catatggctgtcaacagcaagg |
gagtgtcggttaaggagaag |
|
Antxr1 |
157 |
D |
gacgatctccaaagattcgg |
gtaggactctgtggctgatg |
|
Antxr1 |
363 |
C |
gttctgccaggaggagacac |
gcagatggtggatggttcag |
|
Antxr1 |
490 |
C |
aggctctccaaggcattatcc |
ccatcatcgtcttcttcctcac |
|
Antxr1 |
489 |
C |
gctgctctggtggttctg |
gtgttgttcaggggatacttg |
|
Ap2a1 |
510 |
C |
agaacgctatcctctttgagacc |
ggacgtcatcacggttgg |
|
Ap2a1 |
543 |
C |
ggcttttgctgcagacattc |
aggttgggctcactgtcatag |
|
Ap2a1 |
442 |
C |
gttgtcggtgcgcttcc |
catataccagggcaagtccag |
|
Ap2a1 |
531 |
C |
ctatgtgagcgaggaggtgtgg |
cggctgtcatctagggcactg |
|
Ap2a1 |
351 |
C |
agcgggagtcgtccatcttg |
gagctgggtcagccaacaaag |
|
Pak4 |
503 |
C |
aacacatacccacgggctgac |
cgcatgatcaccacctcattg |
|
Pak4 |
477 |
C |
gtacgcgggcacagagttc |
ccgacatgttctcaaattcgtc |
|
Pak4 |
459 |
C |
aggatggggctctcactctg |
cattaggggccatggtatgtg |
|
Pak4 |
412 |
C |
caagcagcaaagacgtgaaactg |
atagggaaggcgggagatgag |
|
Pak4 |
447 |
C |
ctgtccgacttcgggttttgtg |
gtagccaggctctttggttcaagac |
|
|
||||
|
Michaud et al. BMC Genomics 2005 6:164 doi:10.1186/1471-2164-6-164 |
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