Table 3

Experimental analysis of 118 genes from microarray hybridisation
P-value classes
 N° of ORFs
Total ORFs amplified by PCR*
 N° of ORFs positive to PCR amplification
 N° of ORFs analysed by Southern blotting
 N° of ORFs with duplication after Southern hybridisation**
 N° of sequenced ORFs
 N° of ORFs with nucleotide variation in the 70-mer oligo sequence
p < 0.001
 66
 log2-ratio < 0
 19
 19
 7
 7
 -
 -


log2-ratio > 0
 47
 8
 -
 -
 8
 8









p > 0.001
 52
 log2-ratio < 0
 21
 21
 4
 0
 -
 -


log2-ratio > 0
 31
 31
 -
 -
 7
 0

* PCR amplification was carried out with primer anchored to the flanking regions of the gene.

** Determined as higher number of observed fragments after Southern-blot analysis of Rm1021 and of the wild strain.

Giuntini et al. BMC Genomics 2005 6:158   doi:10.1186/1471-2164-6-158